The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis

doi:10.1007/s12020-011-9473-2

	The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis
	Simm, Peter J; Russo, Vincenzo C; Werther, George A
刊名	Endocrine
	2011-08
卷号	40 期号:1 页码:27-34
ISSN号	1559-0100
DOI	10.1007/s12020-011-9473-2
文献子类	Article
英文摘要	While oestrogen is recognized to play a key role in regulating growth, particularly in relation to epiphyseal fusion, the mechanisms that mediate its effects are still unclear. We utilized an in vitro model of chondrogenesis, the RCJ3.1C5.18 cell line, to explore the effect of oestrogen on this process. We demonstrated the presence of oestrogen receptors (ER) α and β in these cells, with increased abundance of both receptor sub-types evident as the cells differentiated. ERα localized to the nucleus, suggesting it was signalling by genomic pathways, while ERβ was seen predominantly in the cytoplasm, suggesting it may be utilizing non-genomic signalling. While exogenous oestrogen had no effect on proliferation or differentiation, we found some evidence for the endogenous production of oestrogen (intracrinology), as suggested by the expression of aromatase in these cells. Selective ERα blockade with methyl piperidinopyrazole (MPP) led to a significant reduction in both proliferation and differentiation, while ERβ blockade with R,R tetrahydrochrysene (THC) led to an increase in these parameters. This is in keeping with results from mouse knockout models suggesting that unopposed ERβ signalling leads to an inhibition of skeletal growth. Our results are further evidence for the importance of differential ER signalling in regulating chondrogenesis. Future studies examining in vivo effects of these agents are required to extrapolate these findings to a mammalian model.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266637]
专题	中国科学院上海药物研究所
通讯作者	Simm, Peter J
作者单位	Department of Endocrinology and Diabetes, Royal Children's Hospital Melbourne, Flemington Rd, Parkville, VIC, 3052, Australia. peter
推荐引用方式 GB/T 7714	Simm, Peter J,Russo, Vincenzo C,Werther, George A. The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis[J]. Endocrine,2011,40(1):27-34.
APA	Simm, Peter J,Russo, Vincenzo C,&Werther, George A.(2011).The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis.Endocrine,40(1),27-34.
MLA	Simm, Peter J,et al."The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis".Endocrine 40.1(2011):27-34.