Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice

doi:10.1038/srep17436

	Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice
	Xu, Ying 2; Ma, Xiao-ping 2; An, Jin-na 2; Zhang, Zi-jia 1; Ding, Jie 2; Qu, Ya-kun 2; Liu, Zhen-li 3; Lin, Na 2
刊名	Scientific reports
	2015-12-08
卷号	5 页码:17436
ISSN号	2045-2322
DOI	10.1038/srep17436
文献子类	Article
英文摘要	The Chinese herbal preparation QiBaoMeiRan formula (QBMR) displayed estrogenic effects in ovariectomized rats after long-term administration in a previous study. The uterus and vagina are negatively influenced by estrogens in hormone therapy. While QBMR is known to be a phytoestrogen, its estrogenic effects and safety on reproductive tissues after short-term administration and its mechanism via estrogen receptor (ER) pathway haven't been studied. Here, we characterized its estrogenic effects using immature mice together with in vitro studies for further molecular characterization. Immature mice were treated with QBMR at doses of 1.125, 2.25, and 4.5 g/kg for 7 days. 1.125 and 2.25 g/kg QBMR promoted the growth and development of uterus and vagina, and upregulated ERα and ERβ expression in reproductive tissues. QBMR had a stimulatory effect on proliferating cell nuclear antigen in vagina but not in uterus, and was without any influence on ki-67 antigen in uterus and vagina. QBMR significantly induced luciferase expression from the ERα/β-estrogen response element (ERE) luciferase reporter and upregulated ERα and ERβ expressions in MCF-7 cells, which were significantly inhibited by estrogen antagonist ICI182,780. This study demonstrated QBMR exerts estrogenic effects on reproductive tissues without side effects and through ER-ERE-dependent pathway.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266528]
专题	中国科学院上海药物研究所
作者单位	1.The MOE Key Laboratory for Standardization of Chinese Medicines and The Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongcheng District Dongzhimen Nanxiao Road 16, Beijing 100700, China; 3.Institute of Basic Theory, China Academy of Chinese Medical Sciences, Dongcheng District Dongzhimen Nanxiao Road 16, Beijing 100700, China
推荐引用方式 GB/T 7714	Xu, Ying,Ma, Xiao-ping,An, Jin-na,et al. Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice[J]. Scientific reports,2015,5:17436.
APA	Xu, Ying.,Ma, Xiao-ping.,An, Jin-na.,Zhang, Zi-jia.,Ding, Jie.,...&Lin, Na.(2015).Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice.Scientific reports,5,17436.
MLA	Xu, Ying,et al."Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice".Scientific reports 5(2015):17436.