Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry

doi:10.1016/j.jchromb.2018.07.034

	Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry
	Xiong, Kai 2,3; Ju, Zhengcai 1; Zhang, Tong 2,4; Wang, Zhengtao 1; Han, Han 2,4
刊名	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
	2018-09-15
卷号	1095 页码:157-165
ISSN号	1873-376X
DOI	10.1016/j.jchromb.2018.07.034
文献子类	Article
英文摘要	Picroside I is an iridoid glycoside derived from Picrorhiza kurroa Royle ex Benth and Picrorhiza scrophulariiflora Pennell and characterized by many biological activities. In this study, a fast, selective, and sensitive UHPLC-MS/MS method was developed and validated to determine picroside I in rat plasma. Analytes were separated by using an ACQUITY UPLC® BEH C18 (2.1 × 50 mm, 1.7 μm) column at a running time of 2 min. Selected reaction monitoring (SRM) transitions were m/z 491.1 → 147.1 for picroside I and m/z 511.1 → 235.1 for the internal standard in a negative ion mode. The established UHPLC-MS/MS method achieved good linearity for picroside I within the range of 0.1-500 ng/mL. The validated method was successfully applied for the pharmacokinetic analysis of picroside I in rats after oral administration. Fifteen metabolites of picroside I were tentatively identified through ultra-high-performance chromatography/tandem quadrupole time-of-flight mass spectrometry, and four metabolites were identified by comparing with the standards. Besides, nine of these metabolites were discovered for the first time. The proposed metabolic pathways of picroside I in vivo can be divided into four parts, namely, phase I reaction of picroside I, including hydroxylation and deoxygenation; phase II reaction of picroside I, including glucuronidation, sulfation, and methylation; phase I biotransformations of metabolites, such as reduction and hydroxylation; and phase II biotransformations of metabolites, such as glucuronidation and sulfation. These results could offer insights into the effectiveness and toxicity of picroside I.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266455]
专题	中国科学院上海药物研究所
通讯作者	Zhang, Tong; Han, Han
作者单位	1.Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201210, China; 2.Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China; 3.School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China; 4.School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China
推荐引用方式 GB/T 7714	Xiong, Kai,Ju, Zhengcai,Zhang, Tong,et al. Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry[J]. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,2018,1095:157-165.
APA	Xiong, Kai,Ju, Zhengcai,Zhang, Tong,Wang, Zhengtao,&Han, Han.(2018).Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,1095,157-165.
MLA	Xiong, Kai,et al."Metabolic profiles and pharmacokinetics of picroside I in rats by liquid chromatography combined with electrospray ionization tandem mass spectrometry".Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 1095(2018):157-165.