Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography

doi:10.1371/journal.pone.0116922

	Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography
	Yang, Yingbo 3; Gu, Lihua 3; Xiao, Ying 2,3; Liu, Qing 1,3; Hu, Haijun 3; Wang, Zhengtao 1,3; Chen, Kaixian3
刊名	PloS one
	2015
卷号	10 期号:2 页码:e0116922
ISSN号	1932-6203
DOI	10.1371/journal.pone.0116922
文献子类	Article
英文摘要	Alpha-glucosidase inhibitors currently form an important basis for developing novel drugs for diabetes treatment. In our preliminary tests, the ethyl acetate fraction of Phlomis tuberosa extracts showed significant α-glucosidase inhibitory activity (IC₅₀ = 100 μg/mL). In the present study, a combined method using Sepbox chromatography and thin-layer chromatography (TLC) bioautography was developed to probe α-glucosidase inhibitors further. The ethyl acetate fraction of P. tuberosa extracts was separated into 150 individual subfractions within 20 h using Sepbox chromatography. Then, under the guidance of TLC bioautography, 20 compounds were successfully isolated from these fractions, including four new diterpenoids [14-hydroxyabieta-8,11,13-triene-11-carbaldehyde-18-oic-12-carboxy-13-(1-hydroxy-1-methylethyl)-lactone (1), 14-hydroxyabieta-8,11,13-triene-17-oic-12-carboxy-13-(1-hydroxy-1-methylethyl)-lactone (2), 14,16-dihydroxyabieta-8,11,13-triene-15,17-dioic acid (3), and phlomisol (15,16-eposy-8,13(16),14-labdatrien-19-ol) (4)], and 16 known compounds. Activity estimation indicated that 15 compounds showed more potent α-glucosidase inhibitory effects (with IC50 values in the range 0.067-1.203 mM) than the positive control, acarbose (IC50 = 3.72 ± 0.113 mM). This is the first report of separation of α-glucosidase inhibitors from P. tuberosa.
语种	英语
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/266395]
专题	中国科学院上海药物研究所
作者单位	1.Shanghai R & D Center for Standardization of Chinese Medicines, Shanghai, China 2.Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, China; 3.The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China;
推荐引用方式 GB/T 7714	Yang, Yingbo,Gu, Lihua,Xiao, Ying,et al. Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography[J]. PloS one,2015,10(2):e0116922.
APA	Yang, Yingbo.,Gu, Lihua.,Xiao, Ying.,Liu, Qing.,Hu, Haijun.,...&Chen, Kaixian.(2015).Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography.PloS one,10(2),e0116922.
MLA	Yang, Yingbo,et al."Rapid identification of α-glucosidase inhibitors from Phlomis tuberosa by Sepbox chromatography and thin-layer chromatography bioautography".PloS one 10.2(2015):e0116922.