A positive feedback regulation of Heme oxygenase 1 by CELF1 in cardiac myoblast cells | |
Liu, Yang5,6; Wang, Huiwen4,6; Wang, Jun5,6; Wei, Bin3; Zhang, Xinyi5,6; Zhang, Mengqi5,6; Cao, Dong2; Dai, Jiang1; Wang, Zhen4; Nyirimigabo, Eric5,6 | |
刊名 | Biochimica et biophysica acta. Gene regulatory mechanisms |
2018-11-30 | |
ISSN号 | 1876-4320 |
DOI | 10.1016/j.bbagrm.2018.11.006 |
文献子类 | Article |
英文摘要 | As an RNA binding protein, CUG-BP Elav-like family (CELF) has been shown to be critical for heart biological functions. However, no reports have revealed the function of CELF1 in hypertrophic cardiomyopathy (HCM). Hinted by RNA immunoprecipitation-sequencing (RIP-seq) data, the influence of the CELF protein on heme oxygenase-1 (HO-1) expression was tested by modulating CELF1 levels. Cardiac hypertrophy is related to oxidative stress-induced damage. Hence, the cardiovascular system may be protected against further injury by upregulating the expression of antioxidant enzymes, such as HO-1. During the past two decades, research has demonstrated the central role of HO-1 in the protection against diseases. Thus, understanding the molecular mechanisms underlying the modulation of HO-1 expression is profoundly important for developing new strategies to prevent cardiac hypertrophy. To elucidate the molecular mechanisms underlying HO-1 regulation by the CELF protein, we performed RNA immunoprecipitation (RIP), biotin pull-down analysis, luciferase reporter and mRNA stability assays. We found that the expression of HO-1 was downregulated by CELF1 through the conserved GU-rich elements (GREs) in HO-1 3'UTR transcripts. Correspondingly, CELF1 expression was regulated by controlling the release of carbon monoxide (CO) in H9C2 cells. The CELF1-HO-1-CO regulation axis constituted a novel positive feedback circuit. In addition, we detected the potential involvement of CELF1 and HO-1 in samples from HCM patients. We found that CELF1 and CELF2, but not HO-1, were highly expressed in HCM heart samples. Thus, a manipulation targeting CELF1 could be developed as a potential therapeutic option for cardiac hypertrophy. |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/266345] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Wang, Huiwen; Ji, Guangju |
作者单位 | 1.Department of Cardiovascular Surgery Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100000, China 2.Laboratory Animal Center, Peking University, Beijing 100871, China; 3.Michigan Surgical Hospital, 21230 Dequindre Road, Warren, MI 48091, USA; 4.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 5.University of the Chinese Academy of Sciences, Beijing 100049, China; 6.National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; |
推荐引用方式 GB/T 7714 | Liu, Yang,Wang, Huiwen,Wang, Jun,et al. A positive feedback regulation of Heme oxygenase 1 by CELF1 in cardiac myoblast cells[J]. Biochimica et biophysica acta. Gene regulatory mechanisms,2018. |
APA | Liu, Yang.,Wang, Huiwen.,Wang, Jun.,Wei, Bin.,Zhang, Xinyi.,...&Ji, Guangju.(2018).A positive feedback regulation of Heme oxygenase 1 by CELF1 in cardiac myoblast cells.Biochimica et biophysica acta. Gene regulatory mechanisms. |
MLA | Liu, Yang,et al."A positive feedback regulation of Heme oxygenase 1 by CELF1 in cardiac myoblast cells".Biochimica et biophysica acta. Gene regulatory mechanisms (2018). |
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