An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration

doi:10.1016/j.biomaterials.2018.11.017

	An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration
	Zhu, Yanlun; Kong, Lingzhi; Farhadi, Fatemeh; Xia, Wei; Chang, Jiang; He, Yaohua; Li, Haiyan
刊名	BIOMATERIALS
	2019-02-01
卷号	192 页码:149
关键词	Osteochondral Continuous stratified scaffolds Injectable hydrogels Co-cultures
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2018.11.017
文献子类	Article
英文摘要	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge. In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration. Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration. The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure. In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by up regulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function. Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed. This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/27367]
专题	中国科学院上海硅酸盐研究所
推荐引用方式 GB/T 7714	Zhu, Yanlun,Kong, Lingzhi,Farhadi, Fatemeh,et al. An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration[J]. BIOMATERIALS,2019,192:149.
APA	Zhu, Yanlun.,Kong, Lingzhi.,Farhadi, Fatemeh.,Xia, Wei.,Chang, Jiang.,...&Li, Haiyan.(2019).An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration.BIOMATERIALS,192,149.
MLA	Zhu, Yanlun,et al."An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration".BIOMATERIALS 192(2019):149.