A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer

doi:10.1016/j.biomaterials.2018.08.048

	A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer
	Wang, Qian 1,2,3; Yan, Hao 3,4; Jin, Yushen3 ; Wang, Zihua 5; Huang, Wenhui 3; Qiu, Jia 3; Kang, Feiyu 4; Wang, Kun3 ; Zhao, Xinming 1,2; Tian, Jie3
刊名	BIOMATERIALS
	2018-11-01
卷号	183 页码:173-184
关键词	Bispecific peptides Early diagnosis Molecular imaging Pancreatic cancer Theranostics
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2018.08.048
通讯作者	Wang, Kun(kun.wang@ia.ac.cn) ; Zhao, Xinming(xinmingzh2017@yeah.net) ; Tian, Jie(jie.tian@ia.ac.cn)
英文摘要	Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest human malignancies with poor patient outcomes often resulting from delayed diagnosis. Therefore, early diagnosis can lead to a better prognosis and improved outcomes. In this study, we have developed a novel conjugate complex of plectin/integrin-targeted bispecific molecular probe, termed Gd-Cy7-PTP/RGD, to be used for magnetic resonance/near-infrared imaging (MRI/NIRF) of pancreatic cancer in vivo. This bispecific molecular probe comprises four parts: Gd(III) for MRI, cyanine 7 (Cy7) for NIRF, the peptide PTP for binding to plectin-1 specifically overexpressed on the surface of PDAC cells, and the peptide RGD for targeting integrin widely expressed on pancreatic duct epithelial cells and angiogenesis. Remarkably, the combination of PTP and RGD greatly increased the targeting efficiency in vitro and in vivo compared to that of either single peptide. Moreover, such bispecific molecular probes target pancreatic neoplasms and angiogenesis simultaneously, producing a "multi-level" targeting effect confirmed by immunofluorescence testing in vitro and in vivo. Under the guidance of MRI/NIRF dual-modality imaging, NIRF-guided delineation of surgical margins during operations was successfully achieved in orthotopic pancreatic cancer. This study promotes further exploration of bispecific molecular probes for clinical application.
资助项目	National Natural Science Foundation of China[81671757] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[61671449] ; National Key Research and Development Program of China[2017YFA0205200] ; National Key Research and Development Program of China[2016YFA0201401] ; National Key Research and Development Program of China[2106YFC0103702] ; CAMS Innovation Fund for Medical Sciences[2016-12M-1-001]
WOS关键词	IN-VIVO ; DUCTAL ADENOCARCINOMA ; INTEGRIN ; THERAPY ; ALPHA-V-BETA-6 ; NANOPARTICLES ; METASTASIS ; STATISTICS ; CARCINOMA ; PATHWAY
WOS研究方向	Engineering ; Materials Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000447116600016
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program of China ; CAMS Innovation Fund for Medical Sciences
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/28140]
专题	中国科学院自动化研究所
通讯作者	Wang, Kun; Zhao, Xinming; Tian, Jie
作者单位	1.Chinese Acad Med Sci, Dept Imaging Diag Natl Canc Ctr, Canc Hosp, Natl Clin Res Ctr Canc, Beijing 100021, Peoples R China 2.Peking Union Med Coll, Beijing 100021, Peoples R China 3.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 4.Tsinghua Univ, Grad Sch Shenzhen, Sch Mat Sci & Engn, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Key Lab Colloid Interface & Chem Thermodynam, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qian,Yan, Hao,Jin, Yushen,et al. A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer[J]. BIOMATERIALS,2018,183:173-184.
APA	Wang, Qian.,Yan, Hao.,Jin, Yushen.,Wang, Zihua.,Huang, Wenhui.,...&Tian, Jie.(2018).A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer.BIOMATERIALS,183,173-184.
MLA	Wang, Qian,et al."A novel plectin/integrin-targeted bispecific molecular probe for magnetic Check tat resonance/near-infrared imaging of pancreatic cancer".BIOMATERIALS 183(2018):173-184.