IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)

doi:10.1016/j.fsi.2019.07.074

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)
	Huang, Baoyu1,2,3,5 ; Tang, Xueying 1,2,3,5; Zhang, Linlin 1,3,5; Li, Li 1,2,4,5; Wang, Wei 1,2,4,5; Liu, Mingkun 1,2,5; Zhang, Guofan 1,2,3,5
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-10-01
卷号	93 页码:551-558
关键词	Oyster Innate immunity IKKe TBK1 NF-kappa B IRF
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.07.074
通讯作者	Li, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gzhang@qdio.ac.cn)
英文摘要	IxB-related kinase epsilon (IKK epsilon) plays a crucial role in the activation of nuclear factor kappa B (NF-kappa B) by phosphorylating inhibitor of NF-kappa B (I kappa B) and in the regulation of interferon (IFN) gene expression by phosphorylating IFN regulatory factors (IRFs). In this study, we cloned an IKK epsilon homologue cDNA (designated as CgIKK epsilon-like) from the Pacific oyster, Crassostrea gigas. The full 2896-bp cDNA sequence comprised a 2163-bp open reading frame (ORF) encoding 720 amino acids. CgIKK epsilon-like is ubiquitously expressed, and its mRNA levels in hemocytes after poly I:C, V. alginolyticus, or OsHV-1 mu Var challenge were analyzed by real-time PCR. Compared to that in the control, CgIKK epsilon-like mRNA expression levels were significantly increased at 3 h and peaked at 6 h after OsHV-1 mu Var challenge; no obvious changes were observed in expression levels until 24 h after either V. alginolyticus or poly I:C challenge, reaching a maximum at 24 h (p < 0.01) and then rapidly decreasing. CgIKK epsilon-like transfection into human cell lines induced NF-kappa B and ISRE activation, while transfection with CgIKK epsilon-like deletion mutants abolished NF-kappa B and ISRE reporter gene activation. Additionally, CgIKK epsilon-like could interact with CgTBK1 and could form homodimers strongly, which may be critical for the immune signaling transduction. Last but not least, we found that CgIKK epsilon-like may increase CgI kappa Bs phosphorylation and could interact with CgIRF8. Together, these results suggest that CgIKK epsilon-like could respond to pathogenic infection, participate in the immune signal transduction and activate NF-kappa B and ISRE reporter genes. Thus, CgIKK epsilon-like could play an important role in the oyster immune system.
资助项目	National Natural Science Foundation of China[31802328] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science[MS2018NO02] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000488998300061
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163250]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Li; Zhang, Guofan
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Huang, Baoyu,Tang, Xueying,Zhang, Linlin,et al. IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:551-558.
APA	Huang, Baoyu.,Tang, Xueying.,Zhang, Linlin.,Li, Li.,Wang, Wei.,...&Zhang, Guofan.(2019).IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas).FISH & SHELLFISH IMMUNOLOGY,93,551-558.
MLA	Huang, Baoyu,et al."IKK epsilon-like plays an important role in the innate immune signaling of the Pacific oyster (Crassostrea gigas)".FISH & SHELLFISH IMMUNOLOGY 93(2019):551-558.