T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis

doi:10.1016/j.fsi.2019.07.021

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	T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis
	Chi, Heng 1,2,3; Sormo, Kristian Gillebo 3; Diao, Jing 2; Dalmo, Roy Ambli 3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2019-10-01
卷号	93 页码:28-38
关键词	Eomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.07.021
通讯作者	Chi, Heng(chiheng@qdio.ac.cn) ; Dalmo, Roy Ambli(roy.dalmo@uit.no)
英文摘要	Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites, Activities of the presumptive regulatory gene were assessed by transfecting different 5'-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from -694 to -376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5'-flanking regions of the cod IFN gamma gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INF gamma reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INF gamma promoter.
资助项目	National Natural Science Foundation of China[31872594] ; National Natural Science Foundation of China[31402326] ; Research Council of Norway[237315] ; Research Council of Norway[239140] ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture[KF201801] ; Tromso Research Foundation ; Key Laboratory of Mariculture (KLM), Ministry of Education, OUC
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000488998300004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/163410]
专题	中国科学院海洋研究所
通讯作者	Chi, Heng; Dalmo, Roy Ambli
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Mariculture, Qingdao 266104, Shandong, Peoples R China 3.UiT Arctic Univ Norway, Fac Biosci Fisheries & Econ, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway
推荐引用方式 GB/T 7714	Chi, Heng,Sormo, Kristian Gillebo,Diao, Jing,et al. T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:28-38.
APA	Chi, Heng,Sormo, Kristian Gillebo,Diao, Jing,&Dalmo, Roy Ambli.(2019).T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis.FISH & SHELLFISH IMMUNOLOGY,93,28-38.
MLA	Chi, Heng,et al."T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis".FISH & SHELLFISH IMMUNOLOGY 93(2019):28-38.