Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII

doi:10.1016/j.bbrc.2019.04.068

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII
	Cheng, J ; Wang, YD ; Pan, YT ; Li, XL ; Hu, J ; Lu, JH
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2019
卷号	513 期号:4 页码:857—861
关键词	COPPER IONS RESOLUTION SUBUNITS
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2019.04.068
文献子类	期刊论文
英文摘要	Blood coagulation factor VIII (FVIII) can bind calcium ions and ion-protein interactions appear central importance for both their structure and function in coagulation cascade. However, the mechanism and details of how calcium dependent structure change of proteins to fulfill their function remain to be fully defined. In this work, PeakForce Quantitative Nanomechanics (PF-QNM) mode atomic force microscopy (AFM) was used to map the topography and mechanical properties of FVIII with single protein resolution under different calcium concentrations. The obtained nanomechanical spectroscopy showed that calcium ions play dual roles in the chain association and structural flexibility of FVIII. Low concentration of calcium ions prefer to bind isolated chains and increase their mechanical properties, whereas they link the heavy and light chains to keep the protein re-association under higher ions concentration. Our results provide a novel insight into the mechanistic details of the metal ions on the stability and function of blood clotting proteins. (C) 2019 Elsevier Inc. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31818]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China; 2.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, ZhangJiang Lab, Shanghai 201210, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 4.Shanghai Univ Med & Hlth Sci, Natl Engn Res Ctr Nanotechnol, Shanghai 201318, Peoples R China
推荐引用方式 GB/T 7714	Cheng, J,Wang, YD,Pan, YT,et al. Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,513(4):857—861.
APA	Cheng, J,Wang, YD,Pan, YT,Li, XL,Hu, J,&Lu, JH.(2019).Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,513(4),857—861.
MLA	Cheng, J,et al."Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 513.4(2019):857—861.