Visualizing glioma margins by real-time tracking of gamma-glutamyltranspeptidase activity

doi:10.1016/j.biomaterials.2018.04.053

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Visualizing glioma margins by real-time tracking of gamma-glutamyltranspeptidase activity
	Liu, YC ; Tan, J ; Zhang, Y ; Zhuang, JF ; Ge, MX ; Shi, B ; Li, J ; Xu, G ; Xu, SC ; Fan, CH
刊名	BIOMATERIALS
	2018
卷号	173 期号:-页码:1-10
关键词	BRAIN-TUMOR SURGERY MALIGNANT GLIOMAS IN-VIVO 5-AMINOLEVULINIC ACID SURGICAL RESECTION PROBES CELLS TRANSFERASE NANOPROBE
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2018.04.053
文献子类	期刊论文
英文摘要	Distinguishing tumor from adjacent non-cancerous tissue can be problematic during surgical treatment of malignant glioma. Consequently, a novel approach to selective discrimination is required. The goal of this study was to determine whether a fluorescent probe activated by gamma-Glutamyltranspeptidase (GGT), an enzyme that is overexpressed on glioma cell membranes but only minimally expressed in normal brain tissue, could be used to visualize glioma margins. Here, we showed that the GGT-activatable fluorescent probe (NC-B-Cys-gamma-Glu) provided real-time in situ tracking of enzyme activity that accurately distinguished glioma from healthy brain tissue. NC-B-Cys-gamma-Glu, which featured distinct ratiometric fluorescence responsiveness after interaction with GGT, enabled monitoring of GGT activity in living cells and differentiation between glioma and normal cells. Topical spraying of NC-B-Cys-gamma-Glu facilitated real-time in vivo identification of orthotopic glioblastomas in a mouse model. Importantly, the tumor, infiltrating area and surrounding normal tissue were distinguished in clinical glioma samples by real-time tracking of GGT activity. When coupled with auto fluorescence bronchoscopy, NC-B-Cys-gamma-Glu offered diagnostic value for cancers overexpressing GGT. Therefore, NC-B-Cys-gamma-Glu might offer a promising tool to guide maximal yet precise tumor resection while sparing non-cancerous tissue. (C) 2018 Elsevier Ltd. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31174]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shandong Univ, Shandong Med Imaging Res Inst, Jinan 250021, Shandong, Peoples R China 2.Shandong Univ, Prov Hosp, Dept Neurosurg, Jinan 250021, Shandong, Peoples R China 3.East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China 4.East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Liu, YC,Tan, J,Zhang, Y,et al. Visualizing glioma margins by real-time tracking of gamma-glutamyltranspeptidase activity[J]. BIOMATERIALS,2018,173(-):1-10.
APA	Liu, YC.,Tan, J.,Zhang, Y.,Zhuang, JF.,Ge, MX.,...&Zhao, CC.(2018).Visualizing glioma margins by real-time tracking of gamma-glutamyltranspeptidase activity.BIOMATERIALS,173(-),1-10.
MLA	Liu, YC,et al."Visualizing glioma margins by real-time tracking of gamma-glutamyltranspeptidase activity".BIOMATERIALS 173.-(2018):1-10.