An ATP-Responsive Linear DNA Hydrogel

doi:10.11777/j.issn1000-3304.2018.18070

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	An ATP-Responsive Linear DNA Hydrogel
	Wang, F ; Zhong, RB ; Tang, Q ; Wang, JB ; Liu, HJ ; Qu, XM ; Wang, LH ; Pei, H
刊名	ACTA POLYMERICA SINICA
	2018
期号	5 页码:553-558
关键词	ELECTROCHEMICAL BIOSENSOR SUPRAMOLECULAR HYDROGEL REVERSIBLE POLYMERS GOLD NANOPARTICLES BUILDING-BLOCKS BLOOD-SERUM APTAMER NANOSTRUCTURES ORIGAMI RELEASE
ISSN号	1000-3304
DOI	10.11777/j.issn1000-3304.2018.18070
文献子类	期刊论文
英文摘要	A single-stranded DNA with adenosine triphosphate (ATP) aptamer sequence and a single-stranded DNA with cohesive end were designed for the formation of linear DNA hydrogel. First, a double-stranded DNA monomer was formed by the hybridization of sticky ends. Then this monomer self-assembled to form a long linear DNA polymer and further form a DNA hydrogel by physically cross-linking The formation of the hydrogel was characterized by rheological tests and the transition from the gel state to the solution state was observed by stress scanning. Using methylene blue (MB) as a marker, the responsive dynamics of the DNA hydrogel to ATP was characterized by UV absorption spectroscopy. Within 15 min after the addition of ATP, the absorbance of the DNA hydrogel at 664 nm rose rapidly and reached a plateau, indicating that the DNA hydrogel can respond quickly to ATP. Moreover, the absorbance of the DNA hydrogel at 664 nm has a good linear correlation with ATP concentration. For comparison, ATP, thymidine triphosphate (TTP), cytidine triphosphate (CTP) and guanosine triphosphate (GTP) were added to the DNA hydrogel, respectively. The UV absorption spectrum test showed that only the ATP-containing DNA hydrogel was depolymerized and MB was released, indicating that the DNA hydrogel has good stability and its response to ATP was specific.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/30837]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
推荐引用方式 GB/T 7714	Wang, F,Zhong, RB,Tang, Q,et al. An ATP-Responsive Linear DNA Hydrogel[J]. ACTA POLYMERICA SINICA,2018(5):553-558.
APA	Wang, F.,Zhong, RB.,Tang, Q.,Wang, JB.,Liu, HJ.,...&Pei, H.(2018).An ATP-Responsive Linear DNA Hydrogel.ACTA POLYMERICA SINICA(5),553-558.
MLA	Wang, F,et al."An ATP-Responsive Linear DNA Hydrogel".ACTA POLYMERICA SINICA .5(2018):553-558.