Stimulated phospholipid synthesis is key for hepatitis B virus replications

doi:10.1038/s41598-019-49367-8

	Stimulated phospholipid synthesis is key for hepatitis B virus replications
	Huang, Qingxia 1,2; Lei, Hehua 2; Ding, Laifeng 1,2; Wang, Yulan 3
刊名	SCIENTIFIC REPORTS
	2019-09-10
卷号	9 页码:9
ISSN号	2045-2322
DOI	10.1038/s41598-019-49367-8
英文摘要	Chronic hepatitis B Virus (HBV) infection has high morbidity, high pathogenicity and unclear pathogenesis. To elucidate the relationship between HBV replication and host phospholipid metabolites, we measured 10 classes of phospholipids in serum of HBV infected patients and cells using ultra performance liquid chromatograph-triple quadruple mass spectrometry. We found that the levels of phosphatidylcholine (PC), phosphatidylethanolamine, and lyso-phosphatidic acid were increased in HBsAg (+) serum of infected patients compared with HBsAg (-), while phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and sphingomyelin were decreased, which were confirmed in an HBV infected HepG2.2.15 cell line. We further evaluated the enzyme levels of PC pathways and found that PCYT1A and LPP1 for PC synthesis were up-regulated after HBV infection. Moreover, HBV replication was inhibited when PCYT1A and LPP1 were inhibited. These results indicated that the PC synthesis in HBV infected host are regulated by PCYT1A and LPP1, which suggests that PCYT1A, LPP1 could be new potential targets for HBV treatment.
资助项目	National Key R&D Program of China[2017YFC0906800] ; National Science Foundation of China[21675169]
WOS关键词	HEPATOCELLULAR-CARCINOMA ; CELL ; ANTIGEN ; BIOSYNTHESIS ; EPIDEMIOLOGY ; INHIBITION ; ACTIVATION ; MECHANISMS ; PARTICLES ; RNAI
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000484986300003
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Science Foundation of China ; National Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14951]
专题	中国科学院武汉物理与数学研究所
通讯作者	Wang, Yulan
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math,Key Lab Magnet Resonance B, Wuhan 430071, Hubei, Peoples R China 3.Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore Phenome Ctr, 59 Nanyang Dr, Singapore, Singapore
推荐引用方式 GB/T 7714	Huang, Qingxia,Lei, Hehua,Ding, Laifeng,et al. Stimulated phospholipid synthesis is key for hepatitis B virus replications[J]. SCIENTIFIC REPORTS,2019,9:9.
APA	Huang, Qingxia,Lei, Hehua,Ding, Laifeng,&Wang, Yulan.(2019).Stimulated phospholipid synthesis is key for hepatitis B virus replications.SCIENTIFIC REPORTS,9,9.
MLA	Huang, Qingxia,et al."Stimulated phospholipid synthesis is key for hepatitis B virus replications".SCIENTIFIC REPORTS 9(2019):9.