The Water Diffusion of Brain Following Hypoglycemia in Rats - A Study with Diffusion Weighted Imaging and Neuropathologic Analysis

doi:10.1016/j.neuroscience.2019.04.036

	The Water Diffusion of Brain Following Hypoglycemia in Rats - A Study with Diffusion Weighted Imaging and Neuropathologic Analysis
	Tong, Fang 1; Zou, Yijuan 2,3; Liang, Yue 1; Lei, Hao 2,3; Lopsong, Tenzin 1; Liu, Yuluo 1; Le Grange, Jehane Michael 4; He, Guanglong 5; Zhou, Yiwu 1
刊名	NEUROSCIENCE
	2019-06-15
卷号	409 页码:58-68
关键词	DWI hypoglycemia neuropathology water diffusion
ISSN号	0306-4522
DOI	10.1016/j.neuroscience.2019.04.036
英文摘要	We established hypoglycemic rat models and divided them into three groups (the sham group, the acute hypoglycemia group and the recovery group). The brain water diffusion was examined using DWI. Thereafter, neuropathologic examinations were performed in order to evaluate the distribution of brain damage. The expression of AQP4 and Caspase3 was also examined using Western blot. We aimed to determine the specific brain regions which were vulnerable to hypoglycemia in relation to the water diffusion and neuropathology. The DWI scanning showed abnormal water diffusion in the cortex, hippocampus and hypothalamus during each stage of hypoglycemia. In the acute hypoglycemia group, the apparent diffusion coefficient (ADC) of the dentate gyrus (DG) and the hypothalamus was increased, while the ADC of the somatosensory cortex (SSc), subcortex and striatum (Str) was decreased. After glucose reperfusion and a 7-day recovery period, most of the hypoglycemia-induced changes in ADC returned to normal, except in the hypothalamus, posterior SSc and DG, which demonstrated increased ADC levels. The lowest AQP4 expression was observed in the cortex of the acute hypoglycemia group. Furthermore, there was increased Caspase3 expression in the hippocampus of the recovery group. The expression of Caspase3 in the hypothalamus was most prominent in the acute hypoglycemia group. Our work revealed that hypoglycemia significantly influenced the water diffusion of the brain. The decrease of AQP4 was associated with the formation of cytotoxic edema in acute hypoglycemia. Hypoglycemia primarily tends to damage the cerebral cortex, hippocampus and hypothalamus and may result in permanent injury to the brain. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.
资助项目	National Key Research and Development Program of China[2018YFC0807203] ; Fundamental Research Funds for the Central Universities, HUST[2016JCTD117] ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics[2016 T151609]
WOS关键词	AQUAPORIN-4 DELETION ; CEREBRAL-ISCHEMIA ; FORENSIC ASPECTS ; EDEMA ; MRI ; AQP4 ; PROGNOSIS ; CASPASES ; NEURONS ; LIGHT
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000470688200006
资助机构	National Key Research and Development Program of China ; National Key Research and Development Program of China ; Fundamental Research Funds for the Central Universities, HUST ; Fundamental Research Funds for the Central Universities, HUST ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Fundamental Research Funds for the Central Universities, HUST ; Fundamental Research Funds for the Central Universities, HUST ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Fundamental Research Funds for the Central Universities, HUST ; Fundamental Research Funds for the Central Universities, HUST ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Fundamental Research Funds for the Central Universities, HUST ; Fundamental Research Funds for the Central Universities, HUST ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14399]
专题	中国科学院武汉物理与数学研究所
通讯作者	He, Guanglong; Zhou, Yiwu
作者单位	1.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, 13 Hangkong Rd, Wuhan 430030, Hubei, Peoples R China 2.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan, Hubei, Peoples R China 4.Huazhong Univ Sci & Technol, Wuhan Union Hosp, Clin Coll 1, Tongji Med Coll, 1277 Jiefang Ave, Wuhan 430022, Hubei, Peoples R China 5.Minist Publ Secur, Inst Forens Sci, 17 Nanli Mulidi, Beijing 100038, Peoples R China
推荐引用方式 GB/T 7714	Tong, Fang,Zou, Yijuan,Liang, Yue,et al. The Water Diffusion of Brain Following Hypoglycemia in Rats - A Study with Diffusion Weighted Imaging and Neuropathologic Analysis[J]. NEUROSCIENCE,2019,409:58-68.
APA	Tong, Fang.,Zou, Yijuan.,Liang, Yue.,Lei, Hao.,Lopsong, Tenzin.,...&Zhou, Yiwu.(2019).The Water Diffusion of Brain Following Hypoglycemia in Rats - A Study with Diffusion Weighted Imaging and Neuropathologic Analysis.NEUROSCIENCE,409,58-68.
MLA	Tong, Fang,et al."The Water Diffusion of Brain Following Hypoglycemia in Rats - A Study with Diffusion Weighted Imaging and Neuropathologic Analysis".NEUROSCIENCE 409(2019):58-68.