Role of Metabolic Activation in Elemicin-Induced Cellular Toxicity

doi:10.1021/acs.jafc.9b02137

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Role of Metabolic Activation in Elemicin-Induced Cellular Toxicity
	Wang, Yi-Kun 2,3; Yang, Xiao-Nan 2,4; Zhu, Xu 2,3; Xiao, Xue-Rong 2; Yang, Xiu-Wei 1; Qin, Hong-Bo2 ; Gonzalez, Frank J.5; Li, Fei 2
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2019-07-24
卷号	67 期号:29 页码:8243-8252
关键词	elemicin l'-hydroxyelemicin metabolic activation cytotoxicity
ISSN号	0021-8561
DOI	10.1021/acs.jafc.9b02137
通讯作者	Qin, Hong-Bo(qinhongbo@mail.kib.ac.cn) ; Li, Fei(lifeib@mail.kib.ac.cn)
英文摘要	Elemicin, an alkenylbenzene constituent of natural oils of several plant species, is widely distributed in food, dietary supplements, and medicinal plants. 1'-Hydroxylation is known to cause metabolic activation of alkenylbenzenes leading to their potential toxicity. The aim of this study was to explore the relationship between elemicin metabolism and its toxicity through comparing the metabolic maps between elemicin and 1'-hydroxyelemicin. Elemicin was transformed into a reactive metabolite of 1'-hydroxyelemicin, which was subsequently conjugated with cysteine (Cys) and N-acetylcysteine (NAC). Administration of NAC could significantly ameliorate the elemicin- and 1'-hydroxyelemicin-induced cytotoxicity of HepG2 cells, while depletion of Cys with diethyl maleate (DEM) increased cytotoxicity. Recombinant human CYP screening and CYP inhibition experiments revealed that multiple CYPs, notably CYP1A1, CYP1A2, and CYP3A4, were responsible for the metabolic activation of elemicin. This study revealed that metabolic activation plays a critical role in elemicin cytotoxicity.
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
WOS记录号	WOS:000477788100020
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/68232]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Qin, Hong-Bo; Li, Fei
作者单位	1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Guangxi Bot Garden Med Plant, Guangxi Key Lab Med Resources Protect & Genet Imp, Nanning 530023, Peoples R China 5.NCI, Lab Metab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Wang, Yi-Kun,Yang, Xiao-Nan,Zhu, Xu,et al. Role of Metabolic Activation in Elemicin-Induced Cellular Toxicity[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(29):8243-8252.
APA	Wang, Yi-Kun.,Yang, Xiao-Nan.,Zhu, Xu.,Xiao, Xue-Rong.,Yang, Xiu-Wei.,...&Li, Fei.(2019).Role of Metabolic Activation in Elemicin-Induced Cellular Toxicity.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(29),8243-8252.
MLA	Wang, Yi-Kun,et al."Role of Metabolic Activation in Elemicin-Induced Cellular Toxicity".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.29(2019):8243-8252.