Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.

doi:10.1016/j.hal.2016.06.003

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 藻类生物学及应用研究中心 > 期刊论文

	Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.
	Li, Xiaochuang 2,3; Li, Shouchun 1; Kong, Renqiu 3; Li, Renhui 3
刊名	HARMFUL ALGAE
	2016-07-01
卷号	57 期号:1 页码:88-97
关键词	Cylindrospermopsis ITS-L Phylogeny Raphidiopsis Taxonomy
ISSN号	1568-9883
DOI	10.1016/j.hal.2016.06.003
英文摘要	The cyanobacterial genera Raphidiopsis and Cylindrospermopsis morphologically resembled each other and are difficult to distinguish, especially when heterocytes do not form in Cylindrospermopsis species. The phylogeny based on multiple gene loci sequences in previous studies cannot divide the strains of these two genera into separate clusters. In the present study, four gene loci 16S rRNA, ITS-L, cpcBA-IGS and rpoC1, sequenced from many Chinese strains (44 Cylindrospermopsis strains and 16 Raphidiopsis strains), were analyzed to infer the phylogenetic relationship between the two genera. According to the 16S rRNA, cpcBA-IGS and rpoC1 gene phylogeny, Cylindrospermopsis and Raphidiopsis strains are intermixed and divide into two or three groups. By contrast, the ITS-L analysis implied that they respectively form two separate and unique clusters. A fragment of 7 bp (RAGAAACT) in ITS-L was conserved for Raphidiopsis strains, which could be distinguished from Cylindrospermopsis strains. A pair of primers targeting the ITS-L fragment specific to Raphidiopsis strains were designed and further verified. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	National Science Foundation China[NSFC 31170189] ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China[2015FB06]
WOS关键词	POPULATION-DYNAMICS ; SUBBA RAJU ; MEDITERRANEA SKUJA ; FLORIDA LAKES ; 1ST REPORT ; RACIBORSKII ; RESERVOIRS ; DOMINANCE ; SEQUENCES ; RELEASE
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000381544600009
资助机构	National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; National Science Foundation China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China ; State Key Laboratory of Freshwater Ecology and Biotechnology Grant, China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/34658]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者	Li, Renhui
作者单位	1.Jiangxi Normal Univ, Coll Life Sci, Nanchang 330027, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,et al. Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.[J]. HARMFUL ALGAE,2016,57(1):88-97.
APA	Li, Xiaochuang,Li, Shouchun,Kong, Renqiu,&Li, Renhui.(2016).Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny..HARMFUL ALGAE,57(1),88-97.
MLA	Li, Xiaochuang,et al."Molecular separation of two long taxonomically debated cyanobacterial genera Cylindrospermopsis and Raphidiopsis (Nostocales) based on the ITS-L phylogeny.".HARMFUL ALGAE 57.1(2016):88-97.