Syntrophic Growth with Direct Interspecies Electron Transfer as the Primary Mechanism for Energy Exchange

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	Syntrophic Growth with Direct Interspecies Electron Transfer as the Primary Mechanism for Energy Exchange
通讯作者	Shrestha, PM (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. pravin@microbio.umass.edu
学科主题	Environmental Sciences & Ecology ; Microbiology
关键词	Metallic-like Conductivity Geobacter-sulfurreducens Pelobacter-carbinolicus Anaerobic-bacteria Fuel-cells Sp-nov Hydrogen Reduction Fe(Iii) Genome
出处	Environmental Microbiology Reports
	2013-09
卷号	5 期号:6 页码:904-910
产权排序	[Shrestha, Pravin Malla; Rotaru, Amelia-Elena; Aklujkar, Muktak; Liu, Fanghua; Shrestha, Minita; Summers, Zarath M.; Malvankar, Nikhil; Flores, Dan Carlo; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA
资助机构	Office of Science (BER), US Department of Energy [DESC0004485]
英文摘要	Direct interspecies electron transfer (DIET) through biological electrical connections is an alternative to interspecies H-2 transfer as a mechanism for electron exchange in syntrophic cultures. However, it has not previously been determined whether electrons received via DIET yield energy to support cell growth. In order to investigate this, co-cultures of Geobacter metallireducens, which can transfer electrons to wild-type G.sulfurreducens via DIET, were established with a citrate synthase-deficient G.sulfurreducens strain that can receive electrons for respiration through DIET only. In a medium with ethanol as the electron donor and fumarate as the electron acceptor, co-cultures with the citrate synthase-deficient G.sulfurreducens strain metabolized ethanol as fast as co-cultures with wild-type, but the acetate that G.metallireducens generated from ethanol oxidation accumulated. The lack of acetate metabolism resulted in less fumarate reduction and lower cell abundance of G.sulfurreducens. RNAseq analysis of transcript abundance was consistent with a lack of acetate metabolism in G.sulfurreducens and revealed gene expression levels for the uptake hydrogenase, formate dehydrogenase, the pilus-associated c-type cytochrome OmcS and pili consistent with electron transfer via DIET. These results suggest that electrons transferred via DIET can serve as the sole energy source to support anaerobic respiration.
内容类型	共享期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6728]
专题	烟台海岸带研究所_科研共享资源
推荐引用方式 GB/T 7714	. Syntrophic Growth with Direct Interspecies Electron Transfer as the Primary Mechanism for Energy Exchange. 2013.