Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室

	Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora
	Jiang DW 1,2; Zhou J 1,3; Yang XW 1; Li J 1; Zhang KQ1; Yang JK1; Bai GZ 1; Xing XJ 1; Tang LY 1; kqzhang1@ynu.edu.cn
刊名	Scientific Reports
	2017
卷号	7 期号:X 页码:e5640
英文摘要	Arthrobotrys oligospora is a typical nematode-trapping fungus. In this study, 37 transformants of A. oligospora were obtained by REMI (restriction enzyme mediated integration) method and phenotypic properties of nine transformants were analyzed. The nine transformants showed differences in growth, conidiation, trap formation, stress tolerance, and/or pathogenicity among each other and with those of the parental wild-type strain (WT). The insertional sites of the hph cassette were identified in transformants X5 and X13. In X5, the cassette was inserted in the non-coding region between AOL_s00076g273 (76g273) and AOL_s00076g274 (76g274) and the transcription of 76g274, but not 76g273, was enhanced in X5. 76g274p had two conserved domains and was predicted as a nucleoprotein, which we confirmed by its nuclear localization in Saccharomyces cerevisiae using the green fluorescent protein-fused 76g274p. The transcription of 76g274 was stimulated or inhibited by several environmental factors. The sporulation yields of 76g274-deficient mutants were decreased by 70%, and transcription of several sporulation-related genes was severely diminished compared to the WT during the conidiation. In summary, a method for screening mutants was established in A. oligospora and using the method, we identified a novel C2H2-type transcription factor that positively regulates the conidiation of A. oligospora.
语种	英语
资助机构	The research described here is jointly supported by the National Basic Research Program of China (2013CB127503), the NSFC- Yunnan Joint Fund (U1402265), the National Natural Science Foundation of China (approved nos. 31272093, 31360019 and 31560025), the Program for Excellent Young Talents of Yunnan University (to Jinkui Yang), and the General Program of the Applied Basic Research Programs of Yunnan Province (approval no. 2016FB044). ; The research described here is jointly supported by the National Basic Research Program of China (2013CB127503), the NSFC- Yunnan Joint Fund (U1402265), the National Natural Science Foundation of China (approved nos. 31272093, 31360019 and 31560025), the Program for Excellent Young Talents of Yunnan University (to Jinkui Yang), and the General Program of the Applied Basic Research Programs of Yunnan Province (approval no. 2016FB044). ; The research described here is jointly supported by the National Basic Research Program of China (2013CB127503), the NSFC- Yunnan Joint Fund (U1402265), the National Natural Science Foundation of China (approved nos. 31272093, 31360019 and 31560025), the Program for Excellent Young Talents of Yunnan University (to Jinkui Yang), and the General Program of the Applied Basic Research Programs of Yunnan Province (approval no. 2016FB044). ; The research described here is jointly supported by the National Basic Research Program of China (2013CB127503), the NSFC- Yunnan Joint Fund (U1402265), the National Natural Science Foundation of China (approved nos. 31272093, 31360019 and 31560025), the Program for Excellent Young Talents of Yunnan University (to Jinkui Yang), and the General Program of the Applied Basic Research Programs of Yunnan Province (approval no. 2016FB044).
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/11940]
专题	昆明动物研究所_动物模型与人类重大疾病机理重点实验室
通讯作者	kqzhang1@ynu.edu.cn; jinkui960@ynu.edu.cn
作者单位	1.State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P. R. China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, P. R. China 3.Panzhihua Institute for Food and Drug Control, Panzhihua, 617000, P. R. China
推荐引用方式 GB/T 7714	Jiang DW,Zhou J,Yang XW,et al. Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora[J]. Scientific Reports,2017,7(X):e5640.
APA	Jiang DW.,Zhou J.,Yang XW.,Li J.,Zhang KQ*.,...&jinkui960@ynu.edu.cn.(2017).Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora.Scientific Reports,7(X),e5640.
MLA	Jiang DW,et al."Random mutagenesis analysis and identification of a novel C2H2-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora".Scientific Reports 7.X(2017):e5640.