Evaluation of novel saponins from Psammosilene tunicoides and their analogs as immunomodulators

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	Evaluation of novel saponins from Psammosilene tunicoides and their analogs as immunomodulators
	ZHANG JIGANG ; Cao WJ(曹文杰) ; TIAN JUNMIAN ; YUE RONGCAI ; LI LI ; GUO BAOYU ; SHAN LEI ; Yu B(俞飚) ; Zhang WD(张卫东)
刊名	Int. Immunopharmacol.
	2012
卷号	14 期号:1 页码:21-26
ISSN号	1567-5769
其他题名	对新型的金铁锁皂甙及其类似物作为免疫调节剂的评价作为免疫调节剂
通讯作者	SHAN LEI ; 俞飚 ; 张卫东
英文摘要	Two original oleanane-type triterpenoid saponins, tunicosaponin A (TSA) and tunicosaponin E (TSE) were isolated from the roots of Psammosilene tunicoides. Four semi-synthetic saponin derivatives, TSA1, TSA2, TSA3, and TSA4, were synthesized from TSA: two derivatives TSE1 and TSE2 were prepared from TSE. Through comparing their hemolytic activity and effects on ovalbumin (OVA)-induced IgG response with those of Quil A, TSA2 was selected as a lead candidate to evaluate acute and hepatotoxic toxicities and adjuvant potentials on the cellular and humoral immune responses of ICR mice against OVA. TSA2 had lower hemolytic activity than Quil A and TSA (P<0.001). Furthermore, TSA2 did not cause any mortality and side effects when mice were administered subcutaneously at a dose up to 1.6 mg. Moreover, no significant hepatotoxic effect was observed in TSA2 groups in doses ranging from 0.05 mg to 0.8 mg. The Con A-, LPS-, and OVA-induced splenocyte proliferation, OVA-specific antibody levels (IgG, IgG1, IgG2a and IgG2b) and IFN-gamma, TNF-alpha, IL-2, IL-4 and IL-5 in serum were significantly enhanced by TSA2 (25 mu g/mouse). The present study of structure-activity relationship indicates that the hydrophobicity of the ester/amide chains bonds to the carboxyl group of the glucuronic acid residue at position C-3 of the triterpene aglycone and the type of sugar chain at C-28 position of saponin could affect the potential of toxicity and adjuvant. Our findings demonstrate that TSA2 possesses higher adjuvant activities with less adverse effects and should be further explored as an immunomodulator for immune responses. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	生命有机化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.intimp.2012.06.006
语种	英语
WOS记录号	WOS:000307693200004
公开日期	2013-08-23
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/28555]
专题	上海有机化学研究所_生命有机化学国家重点实验室
推荐引用方式 GB/T 7714	ZHANG JIGANG,Cao WJ,TIAN JUNMIAN,et al. Evaluation of novel saponins from Psammosilene tunicoides and their analogs as immunomodulators[J]. Int. Immunopharmacol.,2012,14(1):21-26.
APA	ZHANG JIGANG.,曹文杰.,TIAN JUNMIAN.,YUE RONGCAI.,LI LI.,...&张卫东.(2012).Evaluation of novel saponins from Psammosilene tunicoides and their analogs as immunomodulators.Int. Immunopharmacol.,14(1),21-26.
MLA	ZHANG JIGANG,et al."Evaluation of novel saponins from Psammosilene tunicoides and their analogs as immunomodulators".Int. Immunopharmacol. 14.1(2012):21-26.