Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells

doi:10.1667/RR15073.1

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 技术生物与农业工程研究所

	Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells
	Yang, Xiaoyao 1,2; Xu, Shengmin 1,4; Su, Yao 1,2; Chen, Biao 1,2; Yuan, Hang 1,4; Xu, An 1,4; Wu, Lijun 1,3,4
刊名	RADIATION RESEARCH
	2018-11-01
卷号	190 期号:5 页码:494-503
ISSN号	0033-7587
DOI	10.1667/RR15073.1
通讯作者	Xu, Shengmin(shmxu@mail.ustc.edu.cn) ; Wu, Lijun(ljw@ipp.ac.cn)
英文摘要	Connexin molecules are an important component of the gap junction, with connexin43 (Cx43) being the most abundantly expressed type. Src is a nonreceptor tyrosine-protein kinase that affects Cx43 activity by multiple mechanisms. However, it is not clear how Src regulates Cx43 to influence radiation-induced bystander effects (RIBEs). In this study, we demonstrated that Cx43 on Tyr265 was phosphorylated by activated Src in a-irradiated HepG2 cells, with the total expression of Cx43 unchanged. After inhibition of Cx43 phosphorylation in irradiated cells, the frequency of gamma-H2AX foci formation in adjacent nonirradiated bystander cells was significantly enhanced. Furthermore, this study showed that autophagy regulated the activity of Src and phosphorylation of Cx43, and the level of autophagy was correlated with the radiation-induced reactive oxygen species (ROS). These results suggest that ROS and autophagy play an important role in regulating the Src-Cx43 axis to affect the RIBEs. Our findings provide new insights into the Cx43-mediated gap junction intercellular communication, as well as the underlying mechanism of RIBEs. (C) 2018 by Radiation Research Societe
资助项目	International Partnership Program of Chinese Academy of Sciences[116134KYSB20160084] ; National Natural Science Foundation of China[31470829] ; National Natural Science Foundation of China[81273004] ; National Natural Science Foundation of China[31670860] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2016FXCX005] ; ITER program[2014GB112006]
WOS关键词	PROTEIN-KINASE-C ; IONIZING-RADIATION ; TYROSINE PHOSPHORYLATION ; MOLECULAR-BASIS ; V-SRC ; CONNEXIN-43 ; EXPRESSION ; PERMEABILITY ; MECHANISM ; PATHWAY
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	RADIATION RESEARCH SOC
WOS记录号	WOS:000452084900005
资助机构	International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; International Partnership Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; ITER program ; ITER program ; ITER program ; ITER program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/40442]
专题	合肥物质科学研究院_技术生物与农业工程研究所
通讯作者	Xu, Shengmin; Wu, Lijun
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, POB 1138, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China 4.Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xiaoyao,Xu, Shengmin,Su, Yao,et al. Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells[J]. RADIATION RESEARCH,2018,190(5):494-503.
APA	Yang, Xiaoyao.,Xu, Shengmin.,Su, Yao.,Chen, Biao.,Yuan, Hang.,...&Wu, Lijun.(2018).Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells.RADIATION RESEARCH,190(5),494-503.
MLA	Yang, Xiaoyao,et al."Autophagy-Src Regulates Connexin43-Mediated Gap Junction Intercellular Communication in Irradiated HepG2 Cells".RADIATION RESEARCH 190.5(2018):494-503.