Improvement of caproic acid production in a Clostridium kluyveri H068 and Methanogen 166 co-culture fermentation system

doi:10.1186/s13568-018-0705-1

	Improvement of caproic acid production in a Clostridium kluyveri H068 and Methanogen 166 co-culture fermentation system
	Yan, Shoubao 1,2,3; Dong, Dong 1
刊名	AMB EXPRESS
	2018-10-25
卷号	8 页码:13
关键词	Caproic acid production Methanogenic bacteria Co-culture fermentation Scaling-up
ISSN号	2191-0855
DOI	10.1186/s13568-018-0705-1
通讯作者	Yan, Shoubao(1176144756@qq.com)
英文摘要	The aim of this study was to develop a bioprocess capable of producing caproic acid using a binary fermentation system consisting of Clostridium kluyveri H068 and Methanogen 166 which could then be applied to the brewing of Chinese strong flavor liquor. We initially explored the mechanism by which the Methanogen 166 strain facilitates caproic acid accumulation, revealing its ability to convert accumulated H-2 that is produced by C. kluyveri H068 into methane, thereby eliminating the hydrogen-mediated feedback inhibition that normally constrains C. kluyveri H068 and thus enhancing caproic acid production. In addition, laboratory experiments were conducted to optimize this binary fermentation system, allowing us to determine that the optimum conditions for caproic acid production are a mixed inoculum size of 10% with a C. kluyveri H588/Methanogen 166 inoculation ratio of 2:1 (v/v), a sodium acetate concentration of 20g/L, a 4% ethanol content (v/v), and a yeast extract concentration of 10g/L. We further scaled this optimized condition up to use in a 1000 L fermenter and the obtained caproic acid broth was subjected to pit-entry fermentation. Our results demonstrated that this pit-entry fermentation approach was an efficient means of improving the quality of Chinese strong flavor liquor.
资助项目	National Natural Science Funds Fund[31501461] ; Anhui Provincial Natural Science Foundation[1508085QC56] ; Key Research and Development Project of Anhui Province[1804a07020122] ; Key Research and Development Project of Anhui Province[1704f0704067]
WOS关键词	LUZHOU-FLAVOR LIQUOR ; SELECTIVE-INHIBITION ; 2-BROMOETHANESULFONATE ; POPULATIONS ; DIVERSITY ; BACTERIAL ; ACETATE
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGEROPEN
WOS记录号	WOS:000448466200001
资助机构	National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; National Natural Science Funds Fund ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Anhui Provincial Natural Science Foundation ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province ; Key Research and Development Project of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/39885]
专题	中国科学院合肥物质科学研究院
通讯作者	Yan, Shoubao
作者单位	1.Huainan Normal Univ, Sch Life Sci, Huainan 232001, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 3.Anhui Yingjia Grp Co Ltd, Luan 237271, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Yan, Shoubao,Dong, Dong. Improvement of caproic acid production in a Clostridium kluyveri H068 and Methanogen 166 co-culture fermentation system[J]. AMB EXPRESS,2018,8:13.
APA	Yan, Shoubao,&Dong, Dong.(2018).Improvement of caproic acid production in a Clostridium kluyveri H068 and Methanogen 166 co-culture fermentation system.AMB EXPRESS,8,13.
MLA	Yan, Shoubao,et al."Improvement of caproic acid production in a Clostridium kluyveri H068 and Methanogen 166 co-culture fermentation system".AMB EXPRESS 8(2018):13.