isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy

	isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy
	Hu Yechen; Weng Yejing; Jiang Bo; Li Xiao; Zhang Xiaodan; Zhao Baofeng; Wu Qiong; Liang Zhen; Zhang Lihua; Zhang Yukui
刊名	sciencechinachemistry
	2019-01-01
卷号	62 期号:6 页码:708
ISSN号	1674-7291
英文摘要	Protein phosphorylation plays essential roles in various biological procedures. Despite the well-established enrichment strategies for O-phosphoproteomics, the intrinsic acid lability of N-P phosphoramidate bond (phosphorylation of histidine, arginine and lysine) has impaired the progress of N-phosphoproteomics. Herein, we reported a retention time difference combining dimethyl labeling (ReDD) strategy for the isolation and identification of phosphorylated lysine (pLys) peptides. By such a method, pLys peptide could be isolated under 100000-fold interference of non-phosphorylated peptides. Furthermore, ReDD strategy was applied to map pLys sites from E. coli samples, leading to the identification of 11 pLys sites, among which K26p that originating from autonomous glycyl radical cofactor was validated both in mass spectrometry and HPLC co-elution experiments. Furthermore, 112 pLys sites from 100 proteins were identified in HeLa cells. All these results demonstrate that ReDD could provide a first glimpse into Lys phosphorylation, and could be an important step toward the global perspective on protein phosphorylation.
资助项目	[National Key Research and Development Program of China] ; [National Natural Science Foundation of China] ; [CAS Key Project in Frontier Science] ; [DICP, Chinese Academy of Sciences]
语种	英语
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/175057]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Hu Yechen,Weng Yejing,Jiang Bo,et al. isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy[J]. sciencechinachemistry,2019,62(6):708.
APA	Hu Yechen.,Weng Yejing.,Jiang Bo.,Li Xiao.,Zhang Xiaodan.,...&Zhang Yukui.(2019).isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy.sciencechinachemistry,62(6),708.
MLA	Hu Yechen,et al."isolationandidentificationofphosphorylatedlysinepeptidesbyretentiontimedifferencecombiningdimethyllabelingstrategy".sciencechinachemistry 62.6(2019):708.