The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite

doi:10.1039/c9an01119f

	The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite
	Niu, Huan 1,2; Li, Xin 1,2; Peng, Jiaxi 1,2; Zhang, Hongyan 1,2; Zhao, Xingyun 1,2; Zhou, Xiaoyu 1,2; Yu, Dongping 1,2; Liu, Xinyu 1; Wu, Ren'an 1
刊名	ANALYST
	2019-09-07
卷号	144 期号:17 页码:5261-5270
ISSN号	0003-2654
DOI	10.1039/c9an01119f
通讯作者	Wu, Ren'an(wurenan@dicp.ac.cn)
英文摘要	In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from graphene oxide nanosheets and a phenol-formaldehyde polymer via graphitization and KOH activation. In tailoring the large surface area (ca. 2213 m(2) g(-1)) and 3D-layered mesoporous structure, the 3D graphene composite demonstrated not only high efficiency in glycan enrichment but also the size-exclusion effect against residual protein interference. For a standard protein ovalbumin digest, 26 N-linked glycans were identified with good repeatability, and the detection limit was as low as 0.25 ng mu L-1 with the identification of 13 N-linked glycans (S/N > 10). When the mass ratio of the ovalbumin digest to the interfering proteins, i.e., bovine serum albumin and ovalbumin was 1 : 2000 : 2000, 18 N-linked glycans could still be detected with sufficient signal intensities. From a 60 nL minute complex human serum sample, up to 53 N-linked glycans with S/N > 10 were identified after the 3D graphene enrichment, while only 20 N-linked glycans were identified by the porous graphitized carbon material used for comparison. In addition, the application of the 3D graphene composite in profiling the up-regulated and down-regulated N-linked glycans from the real clinical serum samples of ovarian cancer patients confirmed the potential of the 3D graphene composite for analyzing minute and complicated biological samples.
资助项目	National Natural Science Foundation of China[21675156] ; National Natural Science Foundation of China[21375125] ; Instrument Developing Project of the Chinese Academy of Sciences[YZ201503] ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics[DICP TMSR201601]
WOS关键词	SELECTIVE ENRICHMENT ; FACILE PREPARATION ; HIGH-SENSITIVITY ; HIGH-THROUGHPUT ; CARBON ; GLYCOSYLATION ; GLYCOMICS ; CANCER ; OLIGOSACCHARIDES ; GLYCOPROTEOMICS
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000483593000022
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Instrument Developing Project of the Chinese Academy of Sciences ; Instrument Developing Project of the Chinese Academy of Sciences ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics ; Innovation Program of Science and Research from the Dalian Institute of Chemical Physics
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/173309]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Wu, Ren'an
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Niu, Huan,Li, Xin,Peng, Jiaxi,et al. The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite[J]. ANALYST,2019,144(17):5261-5270.
APA	Niu, Huan.,Li, Xin.,Peng, Jiaxi.,Zhang, Hongyan.,Zhao, Xingyun.,...&Wu, Ren'an.(2019).The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite.ANALYST,144(17),5261-5270.
MLA	Niu, Huan,et al."The efficient profiling of serum N-linked glycans by a highly porous 3D graphene composite".ANALYST 144.17(2019):5261-5270.