Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research

doi:10.1002/adma.201902023

	Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research
	Ma, Shujuan 2; Li, Ya 2; Ma, Chen 2; Wang, Yan 2; Ou, Junjie 1,2; Ye, Mingliang 1,2
刊名	ADVANCED MATERIALS
	2019-09-10
页码	27
关键词	affinity chromatography bioseparation glycoproteomics hybrid monoliths monolithic materials phosphoproteomics
ISSN号	0935-9648
DOI	10.1002/adma.201902023
通讯作者	Ou, Junjie(junjieou@dicp.ac.cn)
英文摘要	High-performance liquid chromatography integrated with tandem mass spectrometry (HPLC-MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the "heart" of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow-through pores, are regarded as an alternative to particle-packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next-generation separation materials for high-throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.
资助项目	China State Key Basic Research Program[2017YFA0505004] ; China State Key Basic Research Program[2016YFA0501402] ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS)[DICP TMSR201601] ; National Natural Sciences Foundation of China[21575141] ; CAS-Weigao Research & Development Program[[2017]-009]
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; POROUS POLYMER MONOLITHS ; POLYHEDRAL OLIGOMERIC SILSESQUIOXANE ; POLAR STATIONARY PHASES ; ONE-POT PREPARATION ; LARGE SURFACE-AREA ; LECTIN AFFINITY-CHROMATOGRAPHY ; HIGHLY EFFICIENT SEPARATION ; IMMOBILIZED ENZYME REACTORS ; ENE CLICK REACTION
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000486129600001
资助机构	China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program ; China State Key Basic Research Program ; China State Key Basic Research Program ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; CAS-Weigao Research & Development Program ; CAS-Weigao Research & Development Program
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/173026]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Ou, Junjie
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ma, Shujuan,Li, Ya,Ma, Chen,et al. Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research[J]. ADVANCED MATERIALS,2019:27.
APA	Ma, Shujuan,Li, Ya,Ma, Chen,Wang, Yan,Ou, Junjie,&Ye, Mingliang.(2019).Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research.ADVANCED MATERIALS,27.
MLA	Ma, Shujuan,et al."Challenges and Advances in the Fabrication of Monolithic Bioseparation Materials and their Applications in Proteomics Research".ADVANCED MATERIALS (2019):27.