Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function

doi:10.1016/j.ijbiomac.2019.07.076

	Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function
	Chen, Lijuan 2,3,4; Hao, Jufang 5; Xu, Liangtao 1; Meng, Xiangyu 1; Li, Xiang 1; Nie, Cong 1; Xie, Fuwei 1; Liu, Kejian 1; Peng, Xiaojun 2; Xie, Jianping 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-10-01
卷号	138 页码:29-36
关键词	Carbon nanopartides Cytochrome c Conformation Redox potential Mitochondrial function
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.07.076
通讯作者	Xie, Jianping(ztridicp@126.com)
英文摘要	In this study, we employed multiple spectroscopic methods to analyze the effects of carbon nanoparticles (CNPs) on structure of cytochrome c (Cyt c) and mitochondrial function in plant cells. The tertiary structures of aromatic amino acid in Cyt c were not changed after addition of CNPs. Cyt c was found to be absorbed on the surfaces of CNPs in a non-linear manner and only bound Cyt c can be reduced. In addition, the binding of Cyt c was found to increase the diameter of CNPs at lower concentrations. The redox potential of Cyt c was almost not affected after treatment with CNPs. There were no obvious differences in cellular ATP after exposure to CNPs, and the mitochondrial membrane potential (MMP) was significantly decreased once the CNPs concentration exceeded 31.25 mu g/mL. The levels of reactive oxygen species (ROS) also were increased in BY-2 cells. Taken together, these findings provide basis for the interactions between CNPs and Cyt c, as well as the effect of CNPs treatment on the mitochondria function in plant cells. (C) 2019 Published by Elsevier B.V.
资助项目	College of Chemistry and Molecular Engineering, Zhengzhou University
WOS关键词	SILICA NANOPARTICLES ; INDUCED APOPTOSIS ; GRAPHENE OXIDE ; NANOTUBES ; ACTIVATION ; STABILITY ; KINETICS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000487569000004
资助机构	College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University ; College of Chemistry and Molecular Engineering, Zhengzhou University
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172560]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Xie, Jianping
作者单位	1.Zhengzhou Tobacco Res Inst CNTC, 2 Fengyang Rd, Zhengzhou 450001, Henan, Peoples R China 2.Dalian Univ Technol, State Key Lab Fine Chem, 2 Linggong Rd, Dalian 116024, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 5.Staff Dev Inst China Natl Tobacco Corp, 7 Xinyuan Rd, Zhengzhou 450008, Henan, Peoples R China 6.Beijing Xinna Int Hitech Mat Co Ltd, 14 Kechuang St, Beijing 100076, Peoples R China
推荐引用方式 GB/T 7714	Chen, Lijuan,Hao, Jufang,Xu, Liangtao,et al. Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,138:29-36.
APA	Chen, Lijuan.,Hao, Jufang.,Xu, Liangtao.,Meng, Xiangyu.,Li, Xiang.,...&Guo, Zhijiang.(2019).Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,138,29-36.
MLA	Chen, Lijuan,et al."Spectroscopic approach for the interaction of carbon nanoparticles with cytochrome c and BY-2 cells: Protein structure and mitochondrial function".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 138(2019):29-36.