Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog

doi:10.19756/j.issn.0253-3820.181600

	Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog
	Wang Zhi-Chao 1,2; Liu Jing 2; Piao Hai-Long 1,2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2019
卷号	47 期号:1 页码:59-66
关键词	Capillary electrophoresis-mass spectrometry Cell metabolomics Phosphatase and tensin homolog Metabolic reprogramming Prostate cancer
ISSN号	0253-3820
DOI	10.19756/j.issn.0253-3820.181600
通讯作者	Piao Hai-Long(hpiao@dicp.ac.cn)
英文摘要	Prostate cancer is the most frequently occurred cancer in males. Phosphatase and tensin homolog ( PTEN ) deficiency often occurs in prostate cancer and induces metabolic reprogramming. Metabolic vulnerabilities induced by PTEN deficiency may provide therapeutic targets for cancer therapy. Here, capillary electrophoresis-mass spectrometry ( CE-MS ) based metabolomics analysis was used for analyzing metabolic changes induced by PTEN deficiency in prostate cancer cell DU145 and normal prostate cell RWPE1. 200 and 214 metabolites were detected, and 28 and 37 differential metabolites were authenticated in PTEN knock-downed DU145 and RWPE1 cells compared to their controls , respectively. Threonic acid levels increased , while isobutyrylcarnitine , adenosine diphosphate , N-glycolylneuraminic acid, Asp hypotaurine levels decreased after PTEN silencing in both cell lines. The specific metabolites changes in DU145 after PTEN silencing were L-2-HG, glycerophosphocholine , thiamine, the ratio of GSH to GSSG, and all of them were increased. These metabolites can promote tumor proliferation, metastasis, and resistance to chemotherapy. Creatinine, carnosine and N-acetylneuraminic acid , which had been reported to be biomarkers of cancer diagnosis and prognosis, were regulated by PTEN deficiency. Metabolites changes induced by PTEN deficiency only or combined effect of PTEN deficiency and other cancer-related genes were identified.
资助项目	National Natural Science Foundation of China[81672440] ; National Natural Science Foundation of China[81702771] ; Innovation Program of Science and Research from the DICP, CAS ; DICP[TMSR201601]
WOS关键词	CANCER ; PTEN ; BREAST ; VULNERABILITY ; HYPOXIA
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000470997900007
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovation Program of Science and Research from the DICP, CAS ; Innovation Program of Science and Research from the DICP, CAS ; DICP ; DICP
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/172163]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
通讯作者	Piao Hai-Long
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang Zhi-Chao,Liu Jing,Piao Hai-Long. Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2019,47(1):59-66.
APA	Wang Zhi-Chao,Liu Jing,&Piao Hai-Long.(2019).Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,47(1),59-66.
MLA	Wang Zhi-Chao,et al."Capillary Electrophoresis-Mass Spectrometry Based Metabolomics Analysis of Metabolic Reprogramming Induced by Phosphatase and Tensin Homolog".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 47.1(2019):59-66.