The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis

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	The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis
通讯作者	Zhou, ZH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, 300 Fenglin Rd, Shanghai 200032, Peoples R China.,zhouzhihua@sippe.ac.cn
学科主题	Biotechnology & Applied Microbiology ; Microbiology
关键词	Propionate-degrading Bacterium Rate Anaerobic Bioreactor Chain Fatty-acids Microbial Community Ribosomal-rna Phylogenetic Diversity Methanogenic Archaea Reactor Performance Storage Pits Waste-water
出处	JOURNAL OF APPLIED MICROBIOLOGY
ISSN号	1364-5072
	2009
卷号	106 期号:3 页码:952-966
产权排序	[Liu, F. H.; Wang, S. B.; Zhang, J. S.; Zhang, J.; Yan, X.; Zhou, H. K.; Zhao, G. P.; Zhou, Z. H.] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China; [Liu, F. H.; Zhang, J. S.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
资助机构	Chinese Academy of Sciences [KSCX2-YW-G-022]; National High Technology Research and Development Key Program of China [2007AA021302]; National Natural Science Foundation of China [30700017]
英文摘要	To identify the bacterial and archaeal composition in a mesophilic biogas digester treating pig manure and to compare the consistency of two 16S rDNA-based methods to investigate the microbial structure. Sixty-nine bacterial operational taxonomic units (OTU) and 25 archaeal OTU were identified by sequencing two 16S rDNA clone libraries. Most bacterial OTU were identified as phyla of Firmicutes (47.2% of total clones), Bacteroides (35.4%) and Spirochaetes (13.2%). Methanoculleus bourgensis (29.0%), Methanosarcina barkeri (27.4%) and Methanospirillum hungatei (10.8%) were the dominant methanogens. Only 9% of bacterial and 20% of archaeal OTU matched cultured isolates at a similarity index of >= 97%. About 78% of the dominant bacterial (with abundance > 3%) and 83% of archaeal OTU were recovered from the denaturing gradient gel electrophoresis (DGGE) bands of V3 regions in 16S rDNAs. In the digester, most bacterial and archaeal species were uncultured; bacteria belonging to Firmicutes, Bacteroides and Spirochaetes seem to take charge of cellulolysis, proteolysis, acidogenesis, sulfur-reducing and homoacetogenesis; the most methanogens were typical hydrogenotrophic or hydrogenotrophic/aceticlastic; DGGE profiles reflected the dominant microbiota. This study gave a first insight of the overall microbial structure in a rural biogas digester and also indicated DGGE was useful in displaying its dominant microbiota.
语种	英语
内容类型	共享期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6321]
专题	烟台海岸带研究所_科研共享资源
推荐引用方式 GB/T 7714	. The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. 2009.