Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms

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	Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms
	Shi, Chao 1,2,3; Liu, Yuan2,3 ; Huang, Hui 2; Xia, En-Hua2,3 ; Zhang, Hai-Bin 2,3; Gao, Li-Zhi2
刊名	PLOS ONE
	2013-03-18
卷号	8 期号:3 页码:e59620
关键词	CHLOROPLAST GENOME SEQUENCE COMPLETE NUCLEOTIDE-SEQUENCE INDEPENDENT TRANSFERS HORIZONTAL TRANSFER MAXIMUM-LIKELIHOOD FLOWERING PLANTS RNA-METABOLISM NUCLEUS DNA ORGANIZATION
ISSN号	1932-6203
通讯作者	Gao, LZ (reprint author), Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming, Peoples R China. ; Lgao@mail.kib.ac.cn
英文摘要	Plant chloroplast genes are usually co-transcribed while its posttranscriptional splicing is fairly complex and remains largely unsolved. On basis of sequencing the three complete Camellia (Theaceae) chloroplast genomes for the first time, we comprehensively analyzed the evolutionary patterns of ycf15, a plastid gene quite paradoxical in terms of its function and evolution, along the inferred angiosperm phylogeny. Although many species in separate lineages including the three species reported here contained an intact ycf15 gene in their chloroplast genomes, the phylogenetic mixture of both intact and obviously disabled ycf15 genes imply that they are all non-functional. Both intracellular gene transfer (IGT) and horizontal gene transfer (HGT) failed to explain such distributional anomalies. While, transcriptome analyses revealed that ycf15 was transcribed as precursor polycistronic transcript which contained ycf2, ycf15 and antisense trnL-CAA. The transcriptome assembly was surprisingly found to cover near the complete Camellia chloroplast genome. Many non-coding regions including pseudogenes were mapped by multiple transcripts, indicating the generality of pseudogene transcriptions. Our results suggest that plastid DNA posttranscriptional splicing may involve complex cleavage of nonfunctional genes.
学科主题	Science & Technology - Other Topics
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	CHLOROPLAST GENOME SEQUENCE ; COMPLETE NUCLEOTIDE-SEQUENCE ; INDEPENDENT TRANSFERS ; HORIZONTAL TRANSFER ; MAXIMUM-LIKELIHOOD ; FLOWERING PLANTS ; RNA-METABOLISM ; NUCLEUS ; DNA ; ORGANIZATION
收录类别	SCI
资助信息	Key Project of Natural Science Foundation of Yunnan Province [2010CC011]; Top Talents Program of Yunnan Province [20080A009]; Hundreds Oversea Talents Program of Yunnan Province, Hundreds Talents Program of Chinese Academy of Sciences; Chinese Academy of Science [KSCX2-YW-N-029]; Chinese Department of Science and Technology (973 Program) [2007CB815703]; startup grant of Kunming Institute of Botany, CAS
语种	英语
WOS记录号	WOS:000316411600068
公开日期	2013-06-08
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/16493]
专题	昆明植物研究所_中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species Southwest China, Plant Germplasm & Genom Ctr, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Shi, Chao,Liu, Yuan,Huang, Hui,et al. Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms[J]. PLOS ONE,2013,8(3):e59620.
APA	Shi, Chao,Liu, Yuan,Huang, Hui,Xia, En-Hua,Zhang, Hai-Bin,&Gao, Li-Zhi.(2013).Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms.PLOS ONE,8(3),e59620.
MLA	Shi, Chao,et al."Contradiction between Plastid Gene Transcription and Function Due to Complex Posttranscriptional Splicing: An Exemplary Study of ycf15 Function and Evolution in Angiosperms".PLOS ONE 8.3(2013):e59620.