Axonal bleb recording

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	Axonal bleb recording
	Hu, WQ ; Shu, YS
刊名	NEUROSCIENCE BULLETIN
	2012
卷号	28 期号:4 页码:342-350
关键词	ACTION-POTENTIAL INITIATION NEOCORTICAL PYRAMIDAL NEURONS HIPPOCAMPAL MOSSY FIBERS INACTIVATING K+ CURRENT CELL AXONS GABA(A) RECEPTORS SPINAL-CORD SEGMENT RAT PROPAGATION
ISSN号	1673-7067
通讯作者	Shu, YS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China.,shu@ion.ac.cn
英文摘要	Patch-clamp recording requires direct accessibility of the cell membrane to patch pipettes and allows the investigation of ion channel properties and functions in specific cellular compartments. The cell body and relatively thick dendrites are the most accessible compartments of a neuron, due to their large diameters and therefore great membrane surface areas. However, axons are normally inaccessible to patch pipettes because of their thin structure; thus studies of axon physiology have long been hampered by the lack of axon recording methods. Recently, a new method of patch-clamp recording has been developed, enabling direct and tight-seal recording from cortical axons. These recordings are performed at the enlarged structure (axonal bleb) formed at the cut end of an axon after slicing procedures. This method has facilitated studies of the mechanisms underlying the generation and propagation of the main output signal, the action potential, and led to the finding that cortical neurons communicate not only in action potential-mediated digital mode but also in membrane potential-dependent analog mode.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	973 Program [2011CBA00400]; National Natural Science Foundation of China [31025012]; Shanghai Pujiang Program [07PJ14108]; SA-SIBS Scholarship Program; Hundreds of Talents Program; Strategic Priority Research Program [XDA-01020304]; Chinese Academy of Sciences [KSCX2-YW-R-102]
语种	英语
公开日期	2013-06-04
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2476]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Hu, WQ,Shu, YS. Axonal bleb recording[J]. NEUROSCIENCE BULLETIN,2012,28(4):342-350.
APA	Hu, WQ,&Shu, YS.(2012).Axonal bleb recording.NEUROSCIENCE BULLETIN,28(4),342-350.
MLA	Hu, WQ,et al."Axonal bleb recording".NEUROSCIENCE BULLETIN 28.4(2012):342-350.