Evaluation of alkaloids binding to the parallel quadruplex structure [d(TGGGGT)](4) by electrospray ionization mass spectrometry

	Evaluation of alkaloids binding to the parallel quadruplex structure [d(TGGGGT)](4) by electrospray ionization mass spectrometry
	Xu NS ; Yang HM ; Cui M ; Song FR ; Liu ZQ ; Liu SY
刊名	journal of mass spectrometry
	2012
卷号	47 期号:6 页码:694-700
关键词	CYTOTOXIC PROTOBERBERINE ALKALOIDS POTENT TELOMERASE INHIBITOR DOUBLE-STRANDED DNA BERBERINE DERIVATIVES STABILIZING LIGANDS ETHIDIUM-BROMIDE DISTAMYCIN-A ESI-MS COMPLEXES SEQUENCE
ISSN号	1076-5174
通讯作者	liu sy
中文摘要	in this study, electrospray ionization mass spectrometry (esi-ms) was used to investigate the binding interaction of six alkaloids with parallel intermolecular g-quadruplex [d(tggggt)](4), and five alkaloids including berberine, jatrorrhizine, palmatine, tetrandrine, and fangchinoline showed complexation with the target dna. relative binding affinities were estimated on the basis of mass spectrometric data. the slight differences in chemical structures of berberine, jatrorrhizine, and palmatine had little influence on their binding affinities to [d(tggggt)](4). tetrandrine and fangchinoline selectively bound to [d(tggggt)](4) versus duplex dna. collision-induced dissociation (cid) experiments showed that the complexes with berberine, jatrorrhizine, and palmatine dissociated via strand separation and ligand retaining in the strand while the complexes with tetrandrine and fangchinoline were dissociated via ligand elimination. a comparison of dissociation patterns in cid experiments of complexes with the alkaloids to those with the traditional g-quadruplex dna binders suggested an end-stacking binding mode for tetrandrine and fangchinoline and an intercalation binding mode for berberine, jatrorrhizine, and palmatine to the target dna. the current work not only provides deep insight into alkaloid/[d(tggggt)](4) complexes and useful guidelines for design of efficient anticancer agents but also demonstrates the utility of esi-ms as a powerful tool for evaluating interaction between ligand and quadruplex dna. copyright (c) 2012 john wiley & sons, ltd.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000305528300004
公开日期	2013-05-20
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/48116]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xu NS,Yang HM,Cui M,et al. Evaluation of alkaloids binding to the parallel quadruplex structure [d(TGGGGT)](4) by electrospray ionization mass spectrometry[J]. journal of mass spectrometry,2012,47(6):694-700.
APA	Xu NS,Yang HM,Cui M,Song FR,Liu ZQ,&Liu SY.(2012).Evaluation of alkaloids binding to the parallel quadruplex structure [d(TGGGGT)](4) by electrospray ionization mass spectrometry.journal of mass spectrometry,47(6),694-700.
MLA	Xu NS,et al."Evaluation of alkaloids binding to the parallel quadruplex structure [d(TGGGGT)](4) by electrospray ionization mass spectrometry".journal of mass spectrometry 47.6(2012):694-700.