Neutral red based colorimetric microorganism bioassay for direct toxicity assessment of toxic chemicals in water

	Neutral red based colorimetric microorganism bioassay for direct toxicity assessment of toxic chemicals in water
	Zhai JF ; Liu L ; Yong DM ; Li D ; Dong SJ
刊名	analytical methods
	2012
卷号	4 期号:11 页码:3849-3854
关键词	HEAVY-METAL TOXICITY ESCHERICHIA-COLI MEMBRANE DAMAGE FLOW-CYTOMETRY CELL VIABILITY IN-VITRO ASSAY ADSORPTION CYTOTOXICITY INHIBITION
ISSN号	1759-9660
通讯作者	dong sj
中文摘要	in this work, we develop a reconfigured colorimetric bioassay for direct toxicity assessment (dta) of toxic chemicals in water based on neutral red (nr) as a colorimetric indicator. confocal laser scanning microscopy and uv-vis absorption spectroscopy are employed to prove the feasibility of the proposed assays using p. fluorescens as the model microorganism. the presence of toxicants can damage cells, and this results in an enhanced adsorption of nr by the cells. this fact provides a new strategy for dta of toxic chemicals by comparing the absorption peak intensities of residual nr after incubation in the presence of toxicants. four important toxicants such as 3,5-dichlorophenol (dcp), as3+, hg2+ and cr6+ are tested, and the toxicity order is dcp > hg2+ > cr6+ > as3+, which is identical with that obtained by the ferricyanide-mediated toxicity alerter (fm-tox) assay. the rc50 value of dcp is estimated to be 12.5 mg l-1, which is comparable with that of other dta assays. the responses of e. coli and bod seed (multi-species cultures) to dcp are further examined successfully, suggesting this nr based colorimetric assay may be a general one to measure the response of microorganisms to toxic chemicals. the low cost of nr, easy and simple operation procedure and reliable results make this assay a promising one in dta.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000310281700054
公开日期	2013-04-22
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/47879]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Zhai JF,Liu L,Yong DM,et al. Neutral red based colorimetric microorganism bioassay for direct toxicity assessment of toxic chemicals in water[J]. analytical methods,2012,4(11):3849-3854.
APA	Zhai JF,Liu L,Yong DM,Li D,&Dong SJ.(2012).Neutral red based colorimetric microorganism bioassay for direct toxicity assessment of toxic chemicals in water.analytical methods,4(11),3849-3854.
MLA	Zhai JF,et al."Neutral red based colorimetric microorganism bioassay for direct toxicity assessment of toxic chemicals in water".analytical methods 4.11(2012):3849-3854.