Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products

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	Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products
	LIU WEI ; Wang HJ(王华杰) ; WANG LIPING ; LIU SHANLIN ; Wang JY(王瑾晔)
刊名	BBA-Proteins Proteomics
	2007
卷号	1774 期号:2 页码:258-266
ISSN号	1570-9639
其他题名	Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products
通讯作者	王瑾晔
英文摘要	In the present study, the formation of modified proteins by methyl docosahexaenoate (DHA) peroxidation products in the presence of a metalcatalyzed oxidation system was investigated. Metal-catalyzed oxidation of mixtures containing bovine serum albumin (BSA) and DHA led to formation of two high molecular weight derivatives of BSA. One had a mass of 71.5 kDa as determined by two-dimensional electrophoresis, matrix assisted laser desorption and ionization mass spectrometer (MALDI MS) analysis. The other was estimated to be 93 kDa by SDS-PAGE electrophoresis. The exposure of BSA to DHA also led to the generation of carbonyl groups. Oxygen radical scavengers could inhibit these modifications induced by DHA peroxidation. Furthermore, there was little difference of the peptides mass fingerprinting between the two kinds of modified high-molecular-weight proteins. These results suggest that oxygen radicals formed during lipid peroxidation are involved in the formation of protein derivatives. Our study may be important in the understanding the specific role of docosahexaenoic acid in the formation of modified proteins during aging and its related diseases. (c) 2006 Elsevier B.V All rights reserved.
学科主题	高分子化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.bbapap.2006.11.014
语种	英语
WOS记录号	WOS:000244638900009
公开日期	2013-04-11
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/25463]
专题	上海有机化学研究所_上海有机化学研究所
推荐引用方式 GB/T 7714	LIU WEI,Wang HJ,WANG LIPING,et al. Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products[J]. BBA-Proteins Proteomics,2007,1774(2):258-266.
APA	LIU WEI,王华杰,WANG LIPING,LIU SHANLIN,&王瑾晔.(2007).Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products.BBA-Proteins Proteomics,1774(2),258-266.
MLA	LIU WEI,et al."Formation of high-molecular-weight protein adducts by methyl docosahexaenoate peroxidation products".BBA-Proteins Proteomics 1774.2(2007):258-266.