Cytological and biochemical alterations in Carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Cytological and biochemical alterations in Carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds
	Wu, WZ ; Zhou, BS ; Xu, Y ; Schramm, KW ; Kettrup, A
刊名	ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
	1999-10-01
卷号	37 期号:3 页码:358-363
关键词	YA-ER LAKE
ISSN号	0090-4341
通讯作者	Wu, WZ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Cytological and biochemical alterations of crucial carp (Carassius auratus) hepatocytes were characterized after exposure to sediments from a lake contaminated with dioxins and other industrial chemicals. Carp were exposed in 20 L water containing 25, 50, or 100 g of contaminated sediment for 2 and 4 weeks. Ultrastructural changes in the liver were characterized by severe enlargement of hepatocytes. Alterations in the cell. included formation of condensed and irregular cell nucleus, polynuclei, dispersed heterochromatin, enlargement of the nucleolus, and degeneration of the nucleus. Mitochondrial numbers were reduced and cristae were deformed. Myelin figures and lysosomes were increased, and sometimes cell organelles and cell matrix were totally lost after 4 weeks of exposure. The ultrastructural alterations were correlated with exposure time and sediment concentrations. Hepatosometic index was significantly increased in experimental groups at 2 and 4 weeks as compared with the control group. EROD enzyme activities were strongly induced in liver. A trend from rough endoplasmic reticulum (RER) to SER was observed. Our results suggest that the dioxin-like compounds bound by sediment were bioavailable to C. auratus and cause sublethal effects.
英文摘要	Cytological and biochemical alterations of crucial carp (Carassius auratus) hepatocytes were characterized after exposure to sediments from a lake contaminated with dioxins and other industrial chemicals. Carp were exposed in 20 L water containing 25, 50, or 100 g of contaminated sediment for 2 and 4 weeks. Ultrastructural changes in the liver were characterized by severe enlargement of hepatocytes. Alterations in the cell. included formation of condensed and irregular cell nucleus, polynuclei, dispersed heterochromatin, enlargement of the nucleolus, and degeneration of the nucleus. Mitochondrial numbers were reduced and cristae were deformed. Myelin figures and lysosomes were increased, and sometimes cell organelles and cell matrix were totally lost after 4 weeks of exposure. The ultrastructural alterations were correlated with exposure time and sediment concentrations. Hepatosometic index was significantly increased in experimental groups at 2 and 4 weeks as compared with the control group. EROD enzyme activities were strongly induced in liver. A trend from rough endoplasmic reticulum (RER) to SER was observed. Our results suggest that the dioxin-like compounds bound by sediment were bioavailable to C. auratus and cause sublethal effects.
学科主题	Environmental Sciences; Toxicology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	YA-ER LAKE ; LIVER ULTRASTRUCTURE ; BRACHYDANIO-RERIO ; INDUCTION ; FISH ; TROUT ; PCDDS ; PCDFS ; CHINA ; PCBS
收录类别	SCI
语种	英语
WOS记录号	WOS:000082555500010
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/10176]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.GSF, Natl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
推荐引用方式 GB/T 7714	Wu, WZ,Zhou, BS,Xu, Y,et al. Cytological and biochemical alterations in Carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds[J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,1999,37(3):358-363.
APA	Wu, WZ,Zhou, BS,Xu, Y,Schramm, KW,&Kettrup, A.(1999).Cytological and biochemical alterations in Carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds.ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,37(3),358-363.
MLA	Wu, WZ,et al."Cytological and biochemical alterations in Carassius auratus hepatocytes from exposure to sediment containing dioxins and related compounds".ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 37.3(1999):358-363.