Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically
	Li Yeguang 1; Miao Fengping1,2 ; Geng Yahong 1; Lu Dayan 1; Zhang Chengwu 3; Zeng Mingtao 4
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2012-07-01
卷号	30 期号:4 页码:627-637
关键词	Haematococcus pluvialis astaxanthin quantification spectrophotometry
ISSN号	0254-4059
通讯作者	Li, YG (reprint author), Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China.,yeguang@wbgcas.cn
产权排序	[Li Yeguang; Miao Fengping; Geng Yahong; Lu Dayan] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China; [Miao Fengping] Chinese Acad Sci, Yantai Inst Costal Zone Res, Lab Coastal Zone Bioresources, Yantai 264003, Peoples R China; [Zhang Chengwu] Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China; [Zeng Mingtao] Texas Tech Univ, Dept Biomed Sci, Hlth Sci Ctr, Paul L Foster Sch Med, El Paso, TX 79905 USA
中文摘要	The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.
英文摘要	The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.
学科主题	Limnology; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	FATTY-ACIDS ; PLUVIALIS ; ACCUMULATION ; GREEN ; ESTERS ; HYDROXYLASE ; NITROGEN ; STAGE
收录类别	SCI
资助信息	Yunnan Provincial Sciences and Technology Department, China[2007AD009]; National Natural Science Foundation of China[CNSF30570183]; Knowledge Innovation Program of Chinese Academy of Sciences[KSCX2-YW-G-060]
语种	英语
WOS记录号	WOS:000305906700015
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6091]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Yantai Inst Costal Zone Res, Lab Coastal Zone Bioresources, Yantai 264003, Peoples R China 3.Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China 4.Texas Tech Univ, Dept Biomed Sci, Hlth Sci Ctr, Paul L Foster Sch Med, El Paso, TX 79905 USA
推荐引用方式 GB/T 7714	Li Yeguang,Miao Fengping,Geng Yahong,et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(4):627-637.
APA	Li Yeguang,Miao Fengping,Geng Yahong,Lu Dayan,Zhang Chengwu,&Zeng Mingtao.(2012).Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(4),627-637.
MLA	Li Yeguang,et al."Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.4(2012):627-637.