Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide
	Fu, Xiuli 1,2; Chen, Lingxin1 ; Li, Jinhua1
刊名	ANALYST
	2012
卷号	137 期号:16 页码:3653-3658
关键词	SENSING STRATEGY QUANTIFICATION CHROMATOGRAPHY SENSITIVITY SURFACE SENSOR PROBE ASSAY HG2+
ISSN号	0003-2654
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,lxchen@yic.ac.cn
产权排序	[Fu, Xiuli; Chen, Lingxin; Li, Jinhua] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
中文摘要	A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1).
英文摘要	A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1).
学科主题	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	SENSING STRATEGY ; QUANTIFICATION ; CHROMATOGRAPHY ; SENSITIVITY ; SURFACE ; SENSOR ; PROBE ; ASSAY ; HG2+
收录类别	SCI
资助信息	National Natural Science Foundation of China[20975089, 21105117]; Chinese Academy of Sciences[KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences
语种	英语
WOS记录号	WOS:000306483000009
公开日期	2013-03-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6077]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fu, Xiuli,Chen, Lingxin,Li, Jinhua. Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide[J]. ANALYST,2012,137(16):3653-3658.
APA	Fu, Xiuli,Chen, Lingxin,&Li, Jinhua.(2012).Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide.ANALYST,137(16),3653-3658.
MLA	Fu, Xiuli,et al."Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide".ANALYST 137.16(2012):3653-3658.