The molecular characterization of RNA segment S9 of grass carp hemorrhage virus (GCHV), an aquareovirus

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	The molecular characterization of RNA segment S9 of grass carp hemorrhage virus (GCHV), an aquareovirus
	Qiu, T; Lu, RH; Zhang, J; Zhu, ZY
刊名	AQUACULTURE
	2001-11-26
卷号	203 期号:1-2 页码:1-7
关键词	grass carp hemorrhage virus (GCHV) segment S9 aquareovirus
ISSN号	0044-8486
通讯作者	Lu, RH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Although reovirus infection is one of the major virus diseases of grass carp in China, the available knowledge on the structure and function of genes and proteins of the virus is limited. The complete sequence of the S9 genome segment of grass carp hemorrhage virus (GCHV) was determined. The segment consists of 1130 nucleotides and has a large open reading frame (ORF) encoding a protein of 352 amino acids with predicted molecular mass of 37.7 kDa. Amino acid sequence comparison revealed that the deduced protein encoded by GCHV S9 is closely related to the sigma NS proteins of mammalian reovirus (MRV) and avian reovirus (ARV). Secondary structure analysis displayed that the form of alpha -helices (40.1%) and beta -sheets (49.4%) are the richest two contents in the protein encoded by S9, and this protein is predicted to be a nonstructural protein. (C) 2001 Elsevier Science B.V. All rights reserved.
英文摘要	Although reovirus infection is one of the major virus diseases of grass carp in China, the available knowledge on the structure and function of genes and proteins of the virus is limited. The complete sequence of the S9 genome segment of grass carp hemorrhage virus (GCHV) was determined. The segment consists of 1130 nucleotides and has a large open reading frame (ORF) encoding a protein of 352 amino acids with predicted molecular mass of 37.7 kDa. Amino acid sequence comparison revealed that the deduced protein encoded by GCHV S9 is closely related to the sigma NS proteins of mammalian reovirus (MRV) and avian reovirus (ARV). Secondary structure analysis displayed that the form of alpha -helices (40.1%) and beta -sheets (49.4%) are the richest two contents in the protein encoded by S9, and this protein is predicted to be a nonstructural protein. (C) 2001 Elsevier Science B.V. All rights reserved.
学科主题	Fisheries; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	BLOT HYBRIDIZATION ; PROTEIN ; GENOME ; IDENTIFICATION ; GENOGROUP ; SEQUENCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000172507000001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9912]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Qiu, T,Lu, RH,Zhang, J,et al. The molecular characterization of RNA segment S9 of grass carp hemorrhage virus (GCHV), an aquareovirus[J]. AQUACULTURE,2001,203(1-2):1-7.
APA	Qiu, T,Lu, RH,Zhang, J,&Zhu, ZY.(2001).The molecular characterization of RNA segment S9 of grass carp hemorrhage virus (GCHV), an aquareovirus.AQUACULTURE,203(1-2),1-7.
MLA	Qiu, T,et al."The molecular characterization of RNA segment S9 of grass carp hemorrhage virus (GCHV), an aquareovirus".AQUACULTURE 203.1-2(2001):1-7.