Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development

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	Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development
	Guo, QL; Wang, YP; Jia, WZ; Zhu, ZY
刊名	CHINESE SCIENCE BULLETIN
	2003-08-01
卷号	48 期号:16 页码:1764-1770
关键词	"all-fish" growth hormone gene growth hormone (GH) transgenic carp thymus immunity
ISSN号	1001-6538
通讯作者	Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non-transgenic controls was carried out. The thymus of one-year-old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the unilateral thymus of the controls weighed 20-81 mg with average 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics; well developed with the thickened outer region and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Further analysis with the electron microscopy revealed that pro-liferous cells in the transgenics; were mainly small lymphocytes and no pathological changes were found. The results confirmed that the "All-fish" GH-transgene promotes thymus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH-transgenic carp.
英文摘要	The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non-transgenic controls was carried out. The thymus of one-year-old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the unilateral thymus of the controls weighed 20-81 mg with average 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics; well developed with the thickened outer region and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Further analysis with the electron microscopy revealed that pro-liferous cells in the transgenics; were mainly small lymphocytes and no pathological changes were found. The results confirmed that the "All-fish" GH-transgene promotes thymus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH-transgenic carp.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	RAINBOW-TROUT ; FISH ; GENE ; ENHANCEMENT ; CELLS ; EGGS ; GH
收录类别	SCI
语种	英语
WOS记录号	WOS:000185218200022
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/9656]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Guo, QL,Wang, YP,Jia, WZ,et al. Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development[J]. CHINESE SCIENCE BULLETIN,2003,48(16):1764-1770.
APA	Guo, QL,Wang, YP,Jia, WZ,&Zhu, ZY.(2003).Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development.CHINESE SCIENCE BULLETIN,48(16),1764-1770.
MLA	Guo, QL,et al."Transgene for growth hormone in common carp (Cyprinus carpio L.) promotes thymus development".CHINESE SCIENCE BULLETIN 48.16(2003):1764-1770.