Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)

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	Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)
	Zhao, Xian-Fu ; Liu, Guo-Xiang ; Hu, Zheng-Yu
刊名	CRUSTACEANA
	2006-06-01
卷号	79 期号:6 页码:677-689
关键词	PHOSPHORUS-DEFICIENCY LAKE PHYTOPLANKTON ZOOPLANKTON ALGAE INDICATORS WATER FOOD
ISSN号	0011-216X
通讯作者	Hu, ZY, Chinese Acad Sci, Inst Water Engn Ecol, Minist Water Resources, XiongChu Rd 578, Wuhan 430079, Hubei, Peoples R China
中文摘要	Our studies investigated the physico-chemical properties of alkaline phosphatase excreted by D. magna. This cladoceran mainly released alkaline phosphatase, though it also released a small amount of acid phosphatase. The alkaline phosphatase showed a broad pH optimum (8.05-10.0), and had a broad optimum temperature (30-35 degrees C) with a temperature coefficient (Q(10)) of 2.45. The K-m of the enzyme is 0.15 +/- 0.02 mM when p-nitrophenyl phosphate is used as a substrate, and the V-max is 0.43 +/- 0.01 mu M pNP mg(-1) DW h(-1). Even though alkaline phosphatase had been incubated in chloroform saturated with WC medium for 13 days, its activity was 54% that of the original. The enzyme was strongly inactivated by EDTA, and appeared to be zinc dependent. The alkaline phosphatase activity remained constant when D. magna was fed different quantities of Chlorella sp. The sensitivity of D. magna phosphatase activity to phosphate was time-dependent. During the first 16 hrs, the enzyme was insensitive to phosphate addition, after 24 hrs incubation the enzyme became sensitive to phosphate addition.
英文摘要	Our studies investigated the physico-chemical properties of alkaline phosphatase excreted by D. magna. This cladoceran mainly released alkaline phosphatase, though it also released a small amount of acid phosphatase. The alkaline phosphatase showed a broad pH optimum (8.05-10.0), and had a broad optimum temperature (30-35 degrees C) with a temperature coefficient (Q(10)) of 2.45. The K-m of the enzyme is 0.15 +/- 0.02 mM when p-nitrophenyl phosphate is used as a substrate, and the V-max is 0.43 +/- 0.01 mu M pNP mg(-1) DW h(-1). Even though alkaline phosphatase had been incubated in chloroform saturated with WC medium for 13 days, its activity was 54% that of the original. The enzyme was strongly inactivated by EDTA, and appeared to be zinc dependent. The alkaline phosphatase activity remained constant when D. magna was fed different quantities of Chlorella sp. The sensitivity of D. magna phosphatase activity to phosphate was time-dependent. During the first 16 hrs, the enzyme was insensitive to phosphate addition, after 24 hrs incubation the enzyme became sensitive to phosphate addition.
学科主题	Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	PHOSPHORUS-DEFICIENCY ; LAKE ; PHYTOPLANKTON ; ZOOPLANKTON ; ALGAE ; INDICATORS ; WATER ; FOOD
收录类别	SCI
语种	英语
WOS记录号	WOS:000240232900004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8852]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xian-Fu,Liu, Guo-Xiang,Hu, Zheng-Yu. Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)[J]. CRUSTACEANA,2006,79(6):677-689.
APA	Zhao, Xian-Fu,Liu, Guo-Xiang,&Hu, Zheng-Yu.(2006).Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera).CRUSTACEANA,79(6),677-689.
MLA	Zhao, Xian-Fu,et al."Physico-chemical properties of alkaline phosphatases released by a planktonic crustacean, Daphnia magna (Cladocera)".CRUSTACEANA 79.6(2006):677-689.