A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis

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	A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis
	Zhang L(张立) ; Guo YL(郭寅龙) ; Liu HQ(刘晗青)
刊名	J. Mass Spectrom.
	2004
卷号	39 期号:4 页码:447-457
ISSN号	1076-5174
其他题名	一种新型含碘树脂衍生物的设计、合成方法及其在基于质谱的蛋白质组学研究中的应用
通讯作者	郭寅龙
英文摘要	A novel class of chemically modified iodo-containing resins with isotope-labeled tagging for mass spectrometry-based proteome analysis is described. This iodo-containing resin contains a thiol-reactive group that is used to capture the cysteine (Cys)-containing peptides from peptide mixtures, one 'tag' amino acid, and an aminomethyl polystyrene resin with Rink Amide Linker. The 'tag' amino acid is synthesized in both heavy and light isotope-coded forms and therefore permits the direct relative quantification of peptides/proteins through mass spectrometric analysis. In the iodo-containing resin strategy, the Cys-containing peptides of two samples covalently captured by either light or heavy iodo-containing resin were mixed and washed extensively under stringent conditions. Then the Cys-containing peptides were retrieved by acid-catalyzed elution. Finally, the eluted peptides were directly analyzed by micro liquid chromatography/mass spectrometry for identification and relative quantification. The iodo-containing resins were synthesized by a simple but effective method. Their abilities to identify and quantify the Cys-containing part in two samples were proved by the analysis of mixtures of amino acids, peptides and proteins. Copyright (C) 2004 John Wiley Sons, Ltd.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1002/jms.615
语种	英语
WOS记录号	WOS:000220860200013
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/19862]
专题	上海有机化学研究所_分析化学研究室
推荐引用方式 GB/T 7714	Zhang L,Guo YL,Liu HQ. A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis[J]. J. Mass Spectrom.,2004,39(4):447-457.
APA	张立,郭寅龙,&刘晗青.(2004).A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis.J. Mass Spectrom.,39(4),447-457.
MLA	张立,et al."A novel class of chemically modified iodo-containing resins: design, synthesis and application to mass spectrometry-based proteome analysis".J. Mass Spectrom. 39.4(2004):447-457.