Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)
	Kong, Xianghui 1,2; Wang, Xuzhen 1; Gan, Xiaoni 1; Li, Junbing 1; He, Shunping 1
刊名	PROGRESS IN NATURAL SCIENCE
	2008-02-10
卷号	18 期号:2 页码:155-160
关键词	connective tissue growth factor sequence variation molecular evolution Cyprinidae
ISSN号	1002-0071
通讯作者	He, SP, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要	Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 by indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 by deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	CYTOCHROME-B GENE ; PHYLOGENETIC-RELATIONSHIPS ; SEQUENCE ALIGNMENT ; FISHES ; ACTINOPTERYGII ; ACTIVATION ; INFERENCE ; SOFTWARE ; PACKAGE ; MRBAYES
收录类别	SCI
语种	英语
WOS记录号	WOS:000253767200004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8240]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China
推荐引用方式 GB/T 7714	Kong, Xianghui,Wang, Xuzhen,Gan, Xiaoni,et al. Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)[J]. PROGRESS IN NATURAL SCIENCE,2008,18(2):155-160.
APA	Kong, Xianghui,Wang, Xuzhen,Gan, Xiaoni,Li, Junbing,&He, Shunping.(2008).Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes).PROGRESS IN NATURAL SCIENCE,18(2),155-160.
MLA	Kong, Xianghui,et al."Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei : Cypriniformes)".PROGRESS IN NATURAL SCIENCE 18.2(2008):155-160.