Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus
	Su, Jianguo 1,2; Zhu, Zuoyan 1; Wang, Yaping 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2008-07-01
卷号	25 期号:1-2 页码:106-113
关键词	Gobiocypris rarus Z-DNA binding protein kinase antiviral gene gene cloning characterization
ISSN号	1050-4648
通讯作者	Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Double-stranded RNA-activated protein kinase (PKR) plays an important rote in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In the present study, a PKR-like gene was cloned and characterized from rare minnow Gobiocypris rarus. The full length of the rare minnow PKR-like (GrPKZ) cDNA is 1946 bp in Length and encodes a polypeptide of 503 amino acids with an estimated molecular mass of 57,355 Da and a predicted isoelectric point of 5.83. Analysis of the deduced amino acid sequence indicated that the mature peptide contains two Zalpha domains and one S_TKc domain, and is most similar to the crucian carp (Carassius auratus) PKR-like amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed that GrPKZ mRNA expression is at low levels in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus, GrPKZ expression was up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). Following infection with Aeromonas hydrophila, GrPKZ transcripts were induced at 24 h post-injection (P < 0.05) and returned to control levels at 120 h post-injection. These data imply that GrPKZ is involved in antiviral defense and Toll-like receptor 4 signaling pathway in bacterial infection. (C) 2008 Elsevier Ltd. All rights reserved.
英文摘要	Double-stranded RNA-activated protein kinase (PKR) plays an important rote in interferon-induced antiviral responses, and is also involved in intracellular signaling pathways, including the apoptosis, proliferation, and transcription pathways. In the present study, a PKR-like gene was cloned and characterized from rare minnow Gobiocypris rarus. The full length of the rare minnow PKR-like (GrPKZ) cDNA is 1946 bp in Length and encodes a polypeptide of 503 amino acids with an estimated molecular mass of 57,355 Da and a predicted isoelectric point of 5.83. Analysis of the deduced amino acid sequence indicated that the mature peptide contains two Zalpha domains and one S_TKc domain, and is most similar to the crucian carp (Carassius auratus) PKR-like amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed that GrPKZ mRNA expression is at low levels in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus, GrPKZ expression was up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). Following infection with Aeromonas hydrophila, GrPKZ transcripts were induced at 24 h post-injection (P < 0.05) and returned to control levels at 120 h post-injection. These data imply that GrPKZ is involved in antiviral defense and Toll-like receptor 4 signaling pathway in bacterial infection. (C) 2008 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	PROTEIN-KINASE PKR ; BINDING DOMAINS ; Z-DNA ; GENOME SEQUENCE ; CAB CELLS ; RNA ; INTERFERON ; VIRUS ; REQUIREMENT ; MACROPHAGES
收录类别	SCI
语种	英语
WOS记录号	WOS:000257637600011
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8068]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Dept Aquaculture, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Su, Jianguo,Zhu, Zuoyan,Wang, Yaping. Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2008,25(1-2):106-113.
APA	Su, Jianguo,Zhu, Zuoyan,&Wang, Yaping.(2008).Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,25(1-2),106-113.
MLA	Su, Jianguo,et al."Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 25.1-2(2008):106-113.