Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress

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	Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress
	Wang, Dan; Zhong, Xue-Ping; Qiao, Zhi-Xian; Gui, Jian-Fang
刊名	JOURNAL OF EXPERIMENTAL BIOLOGY
	2008-08-15
卷号	211 期号:16 页码:2700-2706
关键词	Carassius auratus heme oxygenase-1 hypoxia inductive transcription subcellular localization
ISSN号	0022-0949
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Heme oxygenase-1 is the rate-limiting enzyme in the degradation of heme into biliverdin, carbon monoxide and free divalent iron. In this study, we cloned heme oxygenase isoform 1 (CaHO-1) from a hypoxia-tolerant teleost fish Carassius auratus. The full-length cDNA of CaHO-1 is 1247 bp and encodes a protein of 272 amino acids. RT-PCR and real-time PCR analysis indicated that CaHO-1 was predominantly transcribed in posterior kidney, head kidney, gill and intestine, and induction of gene transcription was observed predominantly in posterior kidney under hypoxic stress. Moreover, the hypoxia-induced transcription was confirmed in goldfish larvae and in in vitro cultured CAB cells. Fluorescence of the HO-1-GFP fusion protein revealed a cytoplasmic and plasma membrane localization, which was consistent with the putative transmembrane structure. Subsequently, we established a stably transfected CAB/pcDNA3.1-HO-1 cell line and a control CAB/pcDNA3.1 cell line, and found that the number of dead cells was obviously reduced in the pcDNA3.1-HO-1-transfected group following 4 days of hypoxic (1% O-2) treatment in comparison with numerous detached dead cells in the control pcDNA3.1-transfected cells. Furthermore, a significant cell viability difference between the two kinds of transfected cells during hypoxia-reoxygenation was revealed. Therefore, the data suggest that fish HO-1 might play a significant protective role in cells in response to hypoxic stress.
英文摘要	Heme oxygenase-1 is the rate-limiting enzyme in the degradation of heme into biliverdin, carbon monoxide and free divalent iron. In this study, we cloned heme oxygenase isoform 1 (CaHO-1) from a hypoxia-tolerant teleost fish Carassius auratus. The full-length cDNA of CaHO-1 is 1247 bp and encodes a protein of 272 amino acids. RT-PCR and real-time PCR analysis indicated that CaHO-1 was predominantly transcribed in posterior kidney, head kidney, gill and intestine, and induction of gene transcription was observed predominantly in posterior kidney under hypoxic stress. Moreover, the hypoxia-induced transcription was confirmed in goldfish larvae and in in vitro cultured CAB cells. Fluorescence of the HO-1-GFP fusion protein revealed a cytoplasmic and plasma membrane localization, which was consistent with the putative transmembrane structure. Subsequently, we established a stably transfected CAB/pcDNA3.1-HO-1 cell line and a control CAB/pcDNA3.1 cell line, and found that the number of dead cells was obviously reduced in the pcDNA3.1-HO-1-transfected group following 4 days of hypoxic (1% O(2)) treatment in comparison with numerous detached dead cells in the control pcDNA3.1-transfected cells. Furthermore, a significant cell viability difference between the two kinds of transfected cells during hypoxia-reoxygenation was revealed. Therefore, the data suggest that fish HO-1 might play a significant protective role in cells in response to hypoxic stress.
学科主题	Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	OXIDATIVE STRESS ; CARASSIUS-AURATUS ; GRASS CARP ; EXPRESSION ; CELLS ; DEGRADATION ; ARSENITE ; TISSUES ; VIRUS ; BRAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000258297000020
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/8050]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dan,Zhong, Xue-Ping,Qiao, Zhi-Xian,et al. Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress[J]. JOURNAL OF EXPERIMENTAL BIOLOGY,2008,211(16):2700-2706.
APA	Wang, Dan,Zhong, Xue-Ping,Qiao, Zhi-Xian,&Gui, Jian-Fang.(2008).Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress.JOURNAL OF EXPERIMENTAL BIOLOGY,211(16),2700-2706.
MLA	Wang, Dan,et al."Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress".JOURNAL OF EXPERIMENTAL BIOLOGY 211.16(2008):2700-2706.