Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)

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	Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)
	Hu, Zhengyu 1,2; Li, Yantao 1,3; Sommerfeld, Milton 1; Chen, Feng 3; Hu, Qiang 1
刊名	EUROPEAN JOURNAL OF PHYCOLOGY
	2008
卷号	43 期号:4 页码:365-376
关键词	astaxanthin carotenogenesis flow cytometry Haematococcus pluvialis oxidative stress pigment mutant
ISSN号	0967-0262
通讯作者	Hu, Q, Arizona State Univ, Dept Appl Biol Sci, Polytech Campus,7001 E Williams Field Rd, Mesa, AZ 85212 USA
中文摘要	Many unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis.
英文摘要	Many unicellular green algae can become yellow or red in various natural habitats due to mass accumulation of a secondary carotenoid, such as lutein, or astaxanthin. The accumulation of secondary carotenoids is generally thought to be a survival strategy of the algae under photo-oxidative stress or other adverse environmental conditions. The physiological role of the carotenoids in stress response is less well understood at the subcellular or molecular level. In this study, a stable astaxanthin overproduction mutant (MT 2877) was isolated by chemical mutagenesis of a wild type (WT) of the green microalga Haematococcus pluvialis Flotow NIES-144. MT 2877 was identical to the WT with respect to morphology, pigment composition, and growth kinetics during the early vegetative stage of the life cycle. However, it had the ability to synthesize and accumulate about twice the astaxanthin content of the WT under high light, or under high light in the presence of excess amounts of ferrous sulphate and sodium acetate. Under stress, the mutant exhibited higher photosynthetic activities than the WT, based on considerably higher chlorophyll fluorescence induction, chlorophyll autofluorescence intensities, and oxygen evolution rates. Cell mortality caused by stress was reduced by half in the mutant culture compared with the WT. Enhanced protection of the mutant against stress is attributed to its accelerated carotenogenesis and accumulation of astaxanthin. Our results suggest that MT 2877, or other astaxanthin overproduction Haematococcus mutants, may offer dual benefits, as compared with the wild type, by increasing cellular astaxanthin content while reducing cell mortality during stress-induced carotenogenesis.
学科主题	Plant Sciences; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]	Plant Sciences ; Marine & Freshwater Biology
关键词[WOS]	GIROD ROSTAFINSKI VOLVOCALES ; GREEN-ALGA ; SECONDARY CAROTENOIDS ; PLUVIALIS CHLOROPHYCEAE ; FUNCTIONAL-ASPECTS ; CHLAMYDOMONAS-REINHARDI ; ANTIOXIDANT ROLE ; HIGH IRRADIANCE ; ACCUMULATION ; LACUSTRIS
收录类别	SCI
资助信息	Science Foundation Arizona's Small Business Catalytic Program
语种	英语
WOS记录号	WOS:000261194700004
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7958]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Arizona State Univ, Dept Appl Biol Sci, Mesa, AZ 85212 USA 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430080, Hubei, Peoples R China 3.Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Hu, Zhengyu,Li, Yantao,Sommerfeld, Milton,et al. Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2008,43(4):365-376.
APA	Hu, Zhengyu,Li, Yantao,Sommerfeld, Milton,Chen, Feng,&Hu, Qiang.(2008).Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae).EUROPEAN JOURNAL OF PHYCOLOGY,43(4),365-376.
MLA	Hu, Zhengyu,et al."Enhanced protection against oxidative stress in an astaxanthin-overproduction Haematococcus mutant (Chlorophyceae)".EUROPEAN JOURNAL OF PHYCOLOGY 43.4(2008):365-376.