Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)

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	Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)
	Li, Dunhai 1; Xing, Wei 2; Li, Genbao 1; Liu, Yongding 1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2009-02-01
卷号	21 期号:1 页码:119-125
关键词	Cyanobacterium Cytochemical changes Development Nostoc sphaeroides Polysaccharides
ISSN号	0921-8971
通讯作者	Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	There are several apparent developmental stages in the life cycle of Nostoc sphaeroides Kutzing, an edible cyanobacterium found mainly in paddy fields in central China. The cytochemical changes in developmental stages such as hormogonia, aseriate stage, filamentous stage and colony in N. sphaeroides were examined using fluorescent staining and colorimetric methods. The staining of acidic and sulfated polysaccharides increased with development when hormogonia were used as the starting point. Acidic polysaccharides (AP) were most abundant at the aseriate stage and then decreased, while the sulfated polysaccharides (SP) were highest at the colony stage. Quantitatively, along the developmental process from hormogonia to colony, total carbohydrates first increased, then became stable, and then reached their highest level at the colony stage, while reducing sugars were highest at the hormogonia stage and then decreased sharply once development began. SP were not detectable in the hot water soluble polysaccharides (HWSP), and hormogonia had the lowest content of AP, while old colonies had the highest. The AP content of the aseriate stage, filamentous stage and young colony stage were very similar. The evolutionary relationships reflected in the developmental stages of N. sphaeroides are discussed.
英文摘要	There are several apparent developmental stages in the life cycle of Nostoc sphaeroides Kutzing, an edible cyanobacterium found mainly in paddy fields in central China. The cytochemical changes in developmental stages such as hormogonia, aseriate stage, filamentous stage and colony in N. sphaeroides were examined using fluorescent staining and colorimetric methods. The staining of acidic and sulfated polysaccharides increased with development when hormogonia were used as the starting point. Acidic polysaccharides (AP) were most abundant at the aseriate stage and then decreased, while the sulfated polysaccharides (SP) were highest at the colony stage. Quantitatively, along the developmental process from hormogonia to colony, total carbohydrates first increased, then became stable, and then reached their highest level at the colony stage, while reducing sugars were highest at the hormogonia stage and then decreased sharply once development began. SP were not detectable in the hot water soluble polysaccharides (HWSP), and hormogonia had the lowest content of AP, while old colonies had the highest. The AP content of the aseriate stage, filamentous stage and young colony stage were very similar. The evolutionary relationships reflected in the developmental stages of N. sphaeroides are discussed.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	BLUE-GREEN-ALGAE ; DIFFERENT COLONY MORPHOLOGIES ; STRUCTURAL-CHARACTERIZATION ; NITROGEN-FIXATION ; POLYSACCHARIDES ; COMMUNE ; ULTRAVIOLET ; SCYTONEMIN ; CULTURES ; PIGMENT
收录类别	SCI
资助信息	CAS [KZCX2-YW-426]; National Basic Research program of China [2002CB412306]
语种	英语
WOS记录号	WOS:000263877000015
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7846]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Li, Dunhai,Xing, Wei,Li, Genbao,et al. Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(1):119-125.
APA	Li, Dunhai,Xing, Wei,Li, Genbao,&Liu, Yongding.(2009).Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium).JOURNAL OF APPLIED PHYCOLOGY,21(1),119-125.
MLA	Li, Dunhai,et al."Cytochemical changes in the developmental process of Nostoc sphaeroides (cyanobacterium)".JOURNAL OF APPLIED PHYCOLOGY 21.1(2009):119-125.