Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 期刊论文

	Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella
	Wei, Lili 1,2,3; Sun, Baojian 1,2; Chang, MingXian 1,2; Liu, Yi 1,2; Nie, Pin 1,2
刊名	ENVIRONMENTAL BIOLOGY OF FISHES
	2009-07-01
卷号	85 期号:3 页码:231-238
关键词	Grass carp Immune-relate genes Microcystin-LR Immunotoxicity Quantitative real-time PCR
ISSN号	0378-1909
通讯作者	Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
中文摘要	Recent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.
英文摘要	Recent studies in mammals have revealed that the cyanobacterial toxin MC-LR suppresses immune functions. Nevertheless, immunotoxic effects of microcystins have been little studied in fish. In this paper, we present the profiles of the immune modulation of MC-LR in grass carp, and quantitative real-time PCR methodology was developed for the measurement of relative transcription changes of six immune-related genes in the spleen and head kidney of the grass carp Ctenopharyngodon idella, which were intraperitoneally injected with 50 mu g MC-LR center dot kg(-1) body weight in a three-week period. This study was focused exclusively on gene transcription level changes at different time points after MC-LR exposure, so, only one dose was given. The investigated genes were interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), type I interferon (Type I IFN), peptidoglycan recognition protein-L (PGRP-L), immunoglobulin M (IgM) and major histocompatibility complex class I (MHC-I) genes. The results demonstrated that the transcription levels of the TNF-alpha, type I IFN, and PGRP-L genes in the spleen and head kidney were significantly low at all time points, and those of IL-1 beta were significantly low in the head kidney at different time points. In addition, IgM and MHC-I transcription levels were only significantly low in the spleen and head kidney at 21 d postinjection. The changes in the transcription levels of immune-related genes induced by MC-LR confirmed its effect on inhibiting immune function at the transcription level.
学科主题	Ecology; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Ecology ; Marine & Freshwater Biology
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology
关键词[WOS]	PEPTIDOGLYCAN RECOGNITION PROTEINS ; NECROSIS-FACTOR-ALPHA ; CULTURED RAT HEPATOCYTES ; CYCLIC PEPTIDE TOXIN ; BLUE-GREEN-ALGAE ; TELEOST FISH ; INNATE IMMUNITY ; TUMOR PROMOTER ; CYCLOSPORINE-A ; MESSENGER-RNA
收录类别	SCI
资助信息	National Natural Science Foundation of China [20577065]
语种	英语
WOS记录号	WOS:000267148300008
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7684]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Hubei Province, Peoples R China 3.Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Jianxi Province, Peoples R China
推荐引用方式 GB/T 7714	Wei, Lili,Sun, Baojian,Chang, MingXian,et al. Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella[J]. ENVIRONMENTAL BIOLOGY OF FISHES,2009,85(3):231-238.
APA	Wei, Lili,Sun, Baojian,Chang, MingXian,Liu, Yi,&Nie, Pin.(2009).Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella.ENVIRONMENTAL BIOLOGY OF FISHES,85(3),231-238.
MLA	Wei, Lili,et al."Effects of cyanobacterial toxin microcystin-LR on the transcription levels of immune-related genes in grass carp Ctenopharyngodon idella".ENVIRONMENTAL BIOLOGY OF FISHES 85.3(2009):231-238.