Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)

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	Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
	Chen, Guo-Yuan 1,2; Qiu, Shan-Lian 1,2; Zhou, Yi-Yong 1
刊名	RESEARCH IN MICROBIOLOGY
	2009-04-01
卷号	160 期号:3 页码:173-178
关键词	Ammonia-oxidizing bacteria Ammonia monooxygenase gene Restriction fragment length polymorphism Shallow lakes
ISSN号	0923-2508
通讯作者	Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China
中文摘要	Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH4+) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.
英文摘要	Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(4)(+)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved.
学科主题	Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	16S RIBOSOMAL-RNA ; FRESH-WATER LAKE ; IN-SITU HYBRIDIZATION ; NITRIFYING BACTERIA ; MOLECULAR DIVERSITY ; AMOA ; POPULATIONS ; OXIDATION ; GENE ; NITRIFICATION
收录类别	SCI
资助信息	National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1]
语种	英语
WOS记录号	WOS:000267685500001
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7668]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong. Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)[J]. RESEARCH IN MICROBIOLOGY,2009,160(3):173-178.
APA	Chen, Guo-Yuan,Qiu, Shan-Lian,&Zhou, Yi-Yong.(2009).Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).RESEARCH IN MICROBIOLOGY,160(3),173-178.
MLA	Chen, Guo-Yuan,et al."Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)".RESEARCH IN MICROBIOLOGY 160.3(2009):173-178.