Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity

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	Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity
	Cao, Xiuyun 3; Song, Chunlei 3; Zhou, Yiyong 3; Strojsova, Alena 1,2; Znachor, Petr 1,2; Zapomelova, Eliska 1,2; Vrba, Jaroslav 1,2
刊名	LIMNOLOGY
	2009-08-01
卷号	10 期号:2 页码:95-104
关键词	ELF97 phosphate Phosphorus regeneration Polymictic lakes Algae Heterotrophic flagellates Protozoa
ISSN号	1439-8621
通讯作者	Vrba, J, Univ S Bohemia, Fac Sci, Sadkach 7, Ceske Budejovice 37005, Czech Republic
中文摘要	Extracellular phosphatases are an important part of the phosphorus cycle in aquatic environments. Phosphatase activity (PA) in plankton was studied in seven subtropical shallow lakes of different exploitation management and trophic status in the urban area of Wuhan City. Bulk PA was rather high (range 1.1-11 mu mol l(-1) h(-1)), although concentrations of soluble reactive phosphorus (SRP) were also high (range 27 mu g P l(-1) to similar to 1.5 mg P l(-1)) in all lakes. Cell-associated extracellular PA in phytoplankton was detected using the fluorescence-labelled enzyme activity technique. Phytoplankton species partly contributed to the bulk PA. We found explicit differences in the presence of cell-associated phosphatase within the main phytoplankton groups; species belonging to Chlorophyta and Dinophyta were regularly phosphatase-positive, while Cyanophyta and Bacillariophyceae were phosphatase-negative in all but one case. Furthermore, there is a certain potential of extracellular phosphatases produced by heterotrophic nanoflagellates in most of the lakes. This new finding compromises the 'traditional' interpretation of bulk phosphatase data as being due to overall phytoplankton or bacterial P regeneration.
英文摘要	Extracellular phosphatases are an important part of the phosphorus cycle in aquatic environments. Phosphatase activity (PA) in plankton was studied in seven subtropical shallow lakes of different exploitation management and trophic status in the urban area of Wuhan City. Bulk PA was rather high (range 1.1-11 mu mol l(-1) h(-1)), although concentrations of soluble reactive phosphorus (SRP) were also high (range 27 mu g P l(-1) to similar to 1.5 mg P l(-1)) in all lakes. Cell-associated extracellular PA in phytoplankton was detected using the fluorescence-labelled enzyme activity technique. Phytoplankton species partly contributed to the bulk PA. We found explicit differences in the presence of cell-associated phosphatase within the main phytoplankton groups; species belonging to Chlorophyta and Dinophyta were regularly phosphatase-positive, while Cyanophyta and Bacillariophyceae were phosphatase-negative in all but one case. Furthermore, there is a certain potential of extracellular phosphatases produced by heterotrophic nanoflagellates in most of the lakes. This new finding compromises the 'traditional' interpretation of bulk phosphatase data as being due to overall phytoplankton or bacterial P regeneration.
学科主题	Limnology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Limnology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	PHOSPHORUS-DEFICIENCY INDICATORS ; ENZYME-LABELED FLUORESCENCE ; FRESH-WATER PHYTOPLANKTON ; ALKALINE-PHOSPHATASE ; SPRING PHYTOPLANKTON ; KINETICS ; DONGHU ; ERKEN ; AVAILABILITY ; ENVIRONMENTS
收录类别	SCI
资助信息	National Science Foundation of China [30600088]; Grant Agency of the AS CR [kzcx2-yw-426, A6017202]; Sino-Czech Scientific and Technological Cooperation [36-20]; Czech Ministry of Education, Youth and Sports [M 6007665801, ME 617]
语种	英语
WOS记录号	WOS:000268017800003
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7656]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Univ S Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic 2.Acad Sci Czech Republic, Inst Hydrobiol, Ctr Biol, Ceske Budejovice 37005, Czech Republic 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Cao, Xiuyun,Song, Chunlei,Zhou, Yiyong,et al. Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity[J]. LIMNOLOGY,2009,10(2):95-104.
APA	Cao, Xiuyun.,Song, Chunlei.,Zhou, Yiyong.,Strojsova, Alena.,Znachor, Petr.,...&Vrba, Jaroslav.(2009).Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity.LIMNOLOGY,10(2),95-104.
MLA	Cao, Xiuyun,et al."Extracellular phosphatases produced by phytoplankton and other sources in shallow eutrophic lakes (Wuhan, China): taxon-specific versus bulk activity".LIMNOLOGY 10.2(2009):95-104.