Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus

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	Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus
	Zhu, Rong; Zhang, Yibing; Zhang, Qiya; Gui, Jianfang
刊名	PROGRESS IN NATURAL SCIENCE
	2009-10-10
卷号	19 期号:10 页码:1227-1234
关键词	Double-stranded RNA (dsRNA)-dependent protein kinase (PKR) Inductive expression Paralichthys olivaceus
ISSN号	1002-0071
通讯作者	Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要	The double-stranded RNA (dsRNA)-dependent protein kinase (PKR) belongs to the eIF2 alpha kinase family and plays a critical role in interferon (IFN)-mediated antiviral response. Recently, in Japanese flounder (Paralichthys olivaceus), a PKR gene has been identified. In this study, we showed that PoPKR localized to the cytoplasm, and the dsRNA-binding motifs (dsRBMs) played a determinative role in protein localization. In cultured FEC cells, PoPKR was detected at a low level of constitutive expression but was highly induced after treatment with UV-inactivated grass carp hemorrhagic virus, active SMRV and Poly I:C although with different expression kinetics. In flounder, PoPKR was ubiquitously distributed in all tested tissues, and SMRV infection resulted in significant upregulation at mRNA and protein levels. In order to reveal the role of PoPKR in host antiviral response, its expression upon exposure to various inducers was characterized and further compared with that of PoHRI, which is another eIF2 alpha kinase of flounder. Interestingly, expression comparison revealed that all inducers stimulated upregulation of PoHRI in cultured flounder embryonic cells and fish, with a similar kinetics to PoPKR but to a less extent. These results suggest that, during antiviral immune response, both flounder eIF2 alpha kinases might play similar roles and that PoPKR is the predominant kinase. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	DOUBLE-STRANDED-RNA ; FACTOR 2-ALPHA KINASE ; ENDOPLASMIC-RETICULUM STRESS ; VESICULAR STOMATITIS-VIRUS ; MOLECULAR-CLONING ; BINDING DOMAINS ; EUKARYOTIC INITIATION-FACTOR-2-ALPHA ; FUNCTIONAL-CHARACTERIZATION ; TRANSLATIONAL CONTROL ; VIRAL-INFECTION
收录类别	SCI
资助信息	National Basic Research Program of China [2004CB117403]; High-Tech R & D Program of China [2007AA09Z429, 2007AA09Z423, 2006AA10A402]; National Natural Science Foundation of China [30871922, 30800854]
语种	英语
WOS记录号	WOS:000270021300008
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7572]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Rong,Zhang, Yibing,Zhang, Qiya,et al. Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus[J]. PROGRESS IN NATURAL SCIENCE,2009,19(10):1227-1234.
APA	Zhu, Rong,Zhang, Yibing,Zhang, Qiya,&Gui, Jianfang.(2009).Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus.PROGRESS IN NATURAL SCIENCE,19(10),1227-1234.
MLA	Zhu, Rong,et al."Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus".PROGRESS IN NATURAL SCIENCE 19.10(2009):1227-1234.