Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)

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	Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)
	Xu, Z.1,2; Chang, M. X.1; Qi, Z. T.1; Huang, B.1; Nie, P.1
刊名	VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
	2009-10-15
卷号	131 期号:3-4 页码:268-272
关键词	Joining (J) chain CDNA sequence Expression Chinese soft-shelled turtle Pelodiscus sinensis Bacterial infection
ISSN号	0165-2427
通讯作者	Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
中文摘要	The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved.
英文摘要	The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved.
学科主题	Immunology; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Veterinary Sciences
研究领域[WOS]	Immunology ; Veterinary Sciences
关键词[WOS]	(J)-CHAIN CDNA ; CLONING ; SEQUENCE ; GENE
收录类别	SCI
资助信息	National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010]
语种	英语
WOS记录号	WOS:000270487400013
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7562]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Xu, Z.,Chang, M. X.,Qi, Z. T.,et al. Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,131(3-4):268-272.
APA	Xu, Z.,Chang, M. X.,Qi, Z. T.,Huang, B.,&Nie, P..(2009).Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,131(3-4),268-272.
MLA	Xu, Z.,et al."Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 131.3-4(2009):268-272.