Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio

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	Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio
	Wang, Da 1; Mao, Huiling 2; Peng, Jinxia 1; Li, Xiyin 1; Zhou, Li 1; Gui, Jianfang 1
刊名	PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
	2009-11-10
卷号	19 期号:11 页码:1537-1544
关键词	Gibel carp (Carassius auratus gibelio) Gynogenesis Male-biased mutant family Male-specific SCAR marker (MSM)
ISSN号	1002-0071
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Gibel carp ( Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immuno. fluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
英文摘要	Gibel carp ( Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immuno. fluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology ; Technology
类目[WOS]	Materials Science, Multidisciplinary ; Multidisciplinary Sciences
研究领域[WOS]	Materials Science ; Science & Technology - Other Topics
关键词[WOS]	SEX-DETERMINING REGION ; Y-CHROMOSOME ; FISH ; BLOCH ; GENE ; DIFFERENTIATION ; DNA ; REPRODUCTION ; EVOLUTION ; PROTEIN
收录类别	SCI
资助信息	National Major Basic Research Program [2004CB117401]; National Natural Science Foundation of China [30630050]; Earmarked fund for the Modern Agro-industry Technology Research System ; President Project of the Chinese Academy of Sciences [1072]
语种	英语
WOS记录号	WOS:000271340100011
公开日期	2010-10-13
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/152342/7510]
专题	水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位	1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China
推荐引用方式 GB/T 7714	Wang, Da,Mao, Huiling,Peng, Jinxia,et al. Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio[J]. PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL,2009,19(11):1537-1544.
APA	Wang, Da,Mao, Huiling,Peng, Jinxia,Li, Xiyin,Zhou, Li,&Gui, Jianfang.(2009).Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio.PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL,19(11),1537-1544.
MLA	Wang, Da,et al."Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio".PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL 19.11(2009):1537-1544.